Di(arylamino)aryl compound

ABSTRACT

The present invention provides a compound which is useful as an inhibitor against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins. 
     As a result of extensive and intensive studies on compounds having an inhibitory effect against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins, the inventors of the present invention have found that the di(arylamino)aryl compound of the present invention has inhibitory activity against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins. This finding led to the completion of the present invention. The compound of the present invention can be used as a pharmaceutical composition for preventing and/or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer, etc.

CROSS REFERENCES TO RELATED APPLICATIONS

This application is a Divisional Application of U.S. patent application Ser. No. 12/448,759, filed on Aug. 6, 2009, which was a 371 of International Patent Application No. PCT/JP2008/062188, filed Jul. 4, 2008, and claims priority to Japanese Patent Application No. 2007-178795, filed on Jul. 6, 2007.

TECHNICAL FIELD

The present invention relates to di(arylamino)aryl compounds useful as active ingredients in pharmaceutical compositions, particularly pharmaceutical compositions for cancer therapy.

BACKGROUND ART

Lung cancer is caused by disordered growth of tracheal, bronchial and/or alveolar cells as a result of losing their normal functions. The number of people who die of lung cancer is the largest of the total of cancer deaths (17%), and worldwide about 1.3 million people die of lung cancer each year.

Treatment for lung cancer is divided into three major categories: surgical operation (surgical therapy), anticancer agent (chemotherapy) and radioactive irradiation (radiation therapy), but the effectiveness of treatment will vary depending on the tissue type of lung cancer. For example, although a definite diagnosis of lung cancer is made by a pathologist based on his cytohistopathological diagnosis on a microscope specimen, small cell lung cancer, which constitutes about 20% of lung cancer cases, has often reached an advanced stage at the time of discovery because it generally has a high grade of malignancy and will rapidly grow and spread and will often metastasize to other organs. For this reason, chemotherapy and/or radiation therapy is often used for treatment of this cancer, but the prognosis is poor because small cell lung cancer will often recur although it is relatively sensitive to these therapies. On the other hand, in the case of non-small cell lung cancer, which constitutes the remainder of about 80%, surgical therapy is considered for use until a certain stage, but there is little opportunity to use surgical operation in the subsequent stages where chemotherapy and/or radiation therapy is mainly used for treatment.

Thus, in either type of lung cancer, chemotherapy is an important option for treatment.

EGFR is a receptor tyrosine kinase and, when activated upon ligand binding, causes phosphorylation of tyrosine residues in the receptor's intracellular region and subsequently induces successive activation of cytoplasmic proteins, thereby facilitating cell differentiation and growth (Clinical Cancer Research, 12(18), 2006, p. 5268-5272). EGFR is found to be overexpressed in various malignant tumors (Journal of Cellular Physiology, 194(1), 2003, p. 13-19), and EGFR overexpression is shown to be a factor responsible for bad prognosis in cancer (Annals of Oncology, 15(1), 2004, p. 28-32, Journal of Clinical Oncology, 21(20), 2003, p. 3798-3807). In recent years, EGFR inhibitors have been observed to produce a high clinical effect on a limited population of non-small cell lung cancer patients, and it has been reported that active mutation of EGFR existed in such a patient segment (N. Engl. J. Med. 350, 2004, p. 2129-2139, Science 304, 2004, p. 1497-1500, Proc. Natl. Acad. Sci. 101, 2004, p. 13306-13311). As a result of a conformational change in the ATP-binding site of EGFR, this mutant EGFR is constitutively activated even in the absence of ligand stimulation, and thereby causes canceration of cells. In cancer cells having this mutant EGFR, it is known that they develop apoptosis by the action of gefitinib or erlotinib known as an EGFR inhibitor, resulting in a reduction of the tumor size (Nat. Rev. Cancer 7, 2007, p. 169-181).

ALK (Anaplastic Lymphoma Kinase) is a receptor tyrosine kinase and is a protein having a transmembrane region in the middle part, flanked by a tyrosine kinase region on the carboxyl-terminal side and an extracellular region on the amino-terminal side. It has previously been reported that full-length ALK is expressed in several types of cancer cells of ectodermal origin (e.g., neuroblastoma, glioblastoma, breast cancer, melanoma) (Non-patent Document 1). In some cases of human malignant lymphoma, it has also been reported that the ALK gene is fused with another gene (e.g., NPM gene, CLTCL gene, TFG gene) as a result of chromosomal translocation, and thereby produces an oncogenic fusion tyrosine kinase (Science, vol. 263, p. 1281, 1994; Blood, vol. 86, p. 1954, 1995; Blood, vol. 95, p. 3204, 2000; Blood, vol. 94, p. 3265, 1999). Also in the case of inflammatory myofibroblastic tumor, it is known that the ALK gene is fused with another gene (e.g., CARS gene, SEC31L1 gene) as a result of chromosomal translocation, and thereby produces a fusion tyrosine kinase (Laboratory Investigation, a journal of technical methods and pathology, vol. 83, p. 1255, 2003; International Journal of Cancer, vol. 118, p. 1181, 2006). Most of partner molecules (including EML4 (echinoderm microtubule associated protein like-4)) to be fused with ALK have a complex-forming domain, and the generated fusion products per se also appear to form complexes. This complex formation would induce uncontrol of ALK tyrosine kinase activity and abnormal activation of intracellular signals, thereby causing canceration (Cellular and Molecular Life Science, vol. 61, p. 2939, 2004; Nature Reviews Cancer, vol. 8, p. 11, 2008).

Moreover, recent reports have indicated the presence of a TPM4-ALK fusion protein in esophageal cancer by proteomics analysis procedures (World Journal of Gastroenterology, vol. 12, p. 7104, 2006; Journal Molecular Medicine, vol. 85, p. 863, 2007). Further, after the priority date of the present application, a fusion gene between EML4 and ALK was confirmed in specimens from lung cancer patients, and it was also reported that this EML4-ALK fusion gene has tumorgenicity and is a causal gene of cancer, and that inhibitors against its kinase activity suppress the growth of various cells where the EML4-ALK fusion protein is expressed (Patent Document 1 and Non-patent Document 2). These documents further show that inhibitors of the EML4-ALK fusion protein are useful as therapeutic agents for lung cancer in EML4-ALK polynucleotide-positive lung cancer patients.

Gefitinib and erlotinib mentioned above, which are EGFR inhibitors and are known as useful therapeutic agents for non-small cell lung cancer, have the following chemical structures.

Moreover, Patent Document 1 published after the priority date of the present application shows the following compounds (each being known as an ALK inhibitor) as examples of compounds having inhibitory activity against the EML4-ALK fusion protein, and it also discloses the actual values of their inhibitory activity against the EML4-ALK fusion protein (Patent Document 1). It should be noted that abbreviations for the following compounds are those used in Patent Document 1.

Their respective chemical names are: 4-[(3′-bromo-4′-hydroxyphenyl)amino]-6,7-dimethoxyquinazoline (also called WHI-P154) for compound A; N-[2-(3-chlorophenyl)ethyl]-2-[({[4-(trifluoromethoxy)phenoxy]acetyl}amino)methyl]-1,3-thiazole-4-carboxamide for compound B; 5-chloro-N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}pyrimidine-2,4-diamine for compound C; and 2-[(5-bromo-2-{[2-methoxy-4-(4-methylpiperazin-1-yl)phenyl]amino}pyrimidin-4-yl)amino]-N-methylbenzenesulfonamide for compound D.

Moreover, in ALK fusion protein-expressing lymphoma cells, a compound having ALK inhibitory activity, WHI-P154, has been reported to inhibit cell growth and induce apoptosis (Non-patent Document 3). It should be noted that WHI-P154 is the same as compound A shown above.

Likewise, TAE684 represented by the following formula is known as an inhibitor of a fusion protein from a fusion gene between NPM gene and ALK gene. It should be noted that this compound is the same as compound C shown above.

TAE684 structurally differs from the compounds of the present invention in that the center ring sandwiched between two —NH groups is a chloro-substituted pyrimidine ring.

Moreover, TAE684 has been reported to inhibit the spread of anaplastic large cell lymphoma (ALCL) by its inhibitory activity against the NPM-ALK fusion protein (Non-patent Document 4). On the other hand, although it is described that compounds including TAE684 have inhibitory activity against focal adhesion kinase (FAK) and are thereby useful for preventing and/or treating non-small cell lung cancer and small cell lung cancer, there is no information about actual therapeutic effects on these lung cancers (Patent Document 2).

After the priority date of the present application, further reports were issued showing that ELM4-ALK is expressed in non-small cell lung cancer cells (NCI-H2228), that TFG-ALK is expressed in non-small cell lung cancer patients, and that TAE684 inhibits the growth of non-small cell lung cancer cells (NCI-H2228) (Patent Document 1 and Non-patent Documents 5 and 6).

The supplemental data of Non-patent Document 6 shows that TAE684 has little growth inhibitory activity (inhibition rate: 7.5%) on HCC-827 cells (mutant EGFR protein-expressing cells) under the conditions shown in the document.

Further, after the priority date of the present application, a more recent report has indicated that TAE684 shows growth inhibitory activity on EGFR (L858R mutation)/BaF cells (Non-patent Document 7).

-   Patent Document 1: European Patent Publication No. EP 1914240 -   Patent Document 2: International Publication No. WO 2004/080980 -   Non-patent Document 1: International Journal of Cancer, vol. 100, p.     49, 2002 -   Non-patent Document 2: Nature, vol. 448, no. 2, p. 561, 2007 -   Non-patent Document 3: Laboratory Investigation, vol. 85, p. 1544,     2005 -   Non-patent Document 4: Proceedings of the National Academy of     Science, vol. 104, no. 1, p. 270, 2007 -   Non-patent Document 5: Cell, vol. 131, p. 1190, 2007 -   Non-patent Document 6: Proceedings of the National Academy of     Science, vol. 104, no. 50, p. 19936, 2007 -   Non-patent Document 7: American Association for Cancer Research     Annual Meeting 2008 Proceedings, vol. 49, April 2008, p. 560, #2373

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention

The present invention provides a compound which is useful as an active ingredient in pharmaceutical compositions, particularly pharmaceutical compositions for cancer therapy, and which can be used more safely as an active ingredient in pharmaceutical compositions.

Means for Solving the Problems

As a result of extensive and intensive studies on compounds useful as active ingredients in pharmaceutical compositions for cancer therapy, the inventors of the present invention have found that the di(arylamino)aryl compound of the present invention has excellent inhibitory activity against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins, and is useful as an active ingredient in pharmaceutical compositions for cancer therapy. This finding led to the completion of the present invention.

Namely, the present invention relates to a compound of formula (I) or a salt thereof, as well as a pharmaceutical composition comprising a compound of formula (I) or a salt thereof and an excipient.

(wherein the symbols are as defined below:

—X— represents

(1) a group of formula (II), or

(2) a group of formula (III)

—R⁵ represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl which may be substituted with one or more halogens,

(5) O-lower alkyl which may be substituted with one or more halogens,

(6) —S-lower alkyl,

(7) cyano,

(8) amino which may be substituted with one or two lower alkyls, or

(9) cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls (provided that the triazine ring to which —R⁵ is attached is attached to the nitrogen atom in the cyclic amino),

—R^(6a), —R^(6b), —R^(6c) and —R^(6d), which may be the same or different, each represent

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl, or

(6) cyano,

—W represents

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl,

(6) cyano, or

(7) a group represented by -A-B,

-A- represents

(1) —S(═O)₂—, or

(2) —C(═O)—,

—B represents

(1) lower alkyl,

(2) amino which may be substituted with one or two R^(ZA),

(3) cyclic amino (provided that -A- is attached to the nitrogen atom in the cyclic amino), or

(4) cycloalkyl,

R^(ZA) represents

(1) lower alkyl, or

(2) cycloalkyl, —R^(1a), —R^(1b), —R^(1c) and —R^(1d), which may be the same or different, each represent

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl, or

(6) cyano,

or alternatively

if —W is —H, one of —R^(1a) or —R^(1b) is a group represented by -A-B, and the other of —R^(1a) or —R^(1b), and —R^(1c) and —R^(1d), which may be the same or different, each represent any of (1) to (6) shown above,

—R² represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl which may be substituted with one or more halogens,

(5) O-lower alkyl which may be substituted with one or more halogens,

(6) —S-lower alkyl, or

(7) cyano,

—R³ and —R⁴ are as follows:

(1) one of them represents —H, and the other represents cyclic amino which may be substituted with one or more groups selected from the group consisting of R^(ZB), oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two R^(ZB) (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom in the cyclic amino),

(2) one of them represents —H, and the other represents a group represented by formula (IV) (provided that -L¹ and -L² taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring, wherein if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with R^(ZC))

(3) one of them represents —H, and the other represents a group represented by —Y-Z, or

(4) —R³ and —R⁴ taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring (provided that if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with —CO₂-(lower alkyl which may be substituted with one or more halogens)),

R^(ZB) represents

(1) lower alkyl which may be substituted with one or more groups selected from the group consisting of oxo, —OH, —O-lower alkyl, —S-lower alkyl, halogen and amino which may be substituted with one or two lower alkyls,

R^(ZC) represents

(1) lower alkyl, or

(2) —CO₂-(lower alkyl which may be substituted with phenyl),

—Y— represents

(1) piperidine-1,4-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the piperidine, and —Z is attached to the carbon atom at the 4-position of the piperidine),

(2) piperazine-1,4-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo,

(3) pyrrolidine-1,3-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the pyrrolidine, and —Z is attached to the carbon atom at the 3-position of the pyrrolidine),

(4) azetidine-1,3-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the azetidine, and —Z is attached to the carbon atom at the 3-position of the azetidine),

(5) —O—, or

(6) —N(—R^(ZD))—,

—R^(ZD) represents

(1) —H, or

(2) lower alkyl,

—Z represents

(1) cyclic amino which may be substituted with one or more groups selected from the group consisting of —R^(ZE), oxo, —OH, —O-lower alkyl, phenyl which may be substituted with halogen, piperidin-1-yl, pyrimidin-2-yl, and amino which may be substituted with one or two lower alkyls,

(2) aryl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, and cyano,

(3) cycloalkyl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, cyano, and oxo,

(4) an aromatic heterocyclic ring which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —OH, —O-lower alkyl, and cyano, or

(5) a group of formula (V)

and

—R^(ZE) represents

(1) lower alkyl which may be substituted with one or more groups selected from the group consisting of oxo, —OH, phenyl which may be substituted with halogen, and amino which may be substituted with one or two lower alkyls).

Unless otherwise specified, when symbols used in one chemical formula are also used in another chemical formula, the same symbols have the same meanings.

The present invention also relates to an inhibitor against the kinase activity of mutant EGFR proteins, which comprises a compound of formula (I) or a salt thereof. In a certain embodiment, the present invention relates to an inhibitor against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins.

Moreover, the present invention also relates to a pharmaceutical composition for cancer therapy, which comprises a compound of formula (I) or a salt thereof, i.e., a therapeutic agent for cancer, which comprises a compound of formula (I) or a salt thereof.

Moreover, the present invention also relates to the use of a compound of formula (I) or a salt thereof for the manufacture of a pharmaceutical composition for cancer therapy, the use of a compound of formula (I) or a salt thereof for cancer therapy, as well as a method for cancer therapy, which comprises administering an effective amount of a compound of formula (I) or a salt thereof to a patient.

Advantages of the Invention

The compound of formula (I) or a salt thereof has inhibitory activity against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins, as well as growth inhibitory activity against human non-small cell lung cancer cell lines NCI-H2228 and HCC827, and can be used as an active ingredient in pharmaceutical compositions for preventing and/or treating cancer, such as lung cancer in one embodiment, non-small cell lung cancer or small cell lung cancer in another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer in yet another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer in yet another embodiment, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer in yet another embodiment.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the results of the screening for EML4-ALK fusion polynucleotide in specimens of lung cancer patients. Lane “46, XX” shows the result of using peripheral monocytes of a normal healthy female subject, and “ID #2” to “ID #42” show the result of using samples obtained from excised specimens from lung cancer patients. In addition, lane “NTC” shows the result without added substrate cDNA. Lane “marker” is the lane where the size marker DNA was electrophoresed (upper section). The results of amplification of GAPDH cDNA are shown in the lower section. Sex (M, male; F, female), pathology (S, squamous cell carcinoma; A, adenocarcinoma; AS, adenosquamous carcinoma; B, bronchiolo-aleveolar carcinoma) and the presence or absence of EGFR mutation and the presence or absence of smoking history are shown in the upper part of the figure.

FIG. 2 shows tumorgenicity of the genes. The upper section of the figure (3T3) shows 3T3 fibroblast cells when a blank vector (Vector), and expression plasmid such as full-length ALK/pMXS (ALK), EML4-ALKv1/pMXS (EML4-ALK) or EML4-ALK (K589M)/pMXS were introduced. The scale bar represents 100 μm. The lower section of the figure (Nude mice) shows the result of the inoculation of each 3T3 fibroblast cell line to nude mice.

BEST MODE FOR CARRYING OUT THE INVENTION

The present invention provides the following.

[1] A compound of formula (I) or a salt thereof:

(wherein the symbols are as defined below:

—X— represents

(1) a group of formula (II), or

(2) a group of formula (III)

—R⁵ represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl which may be substituted with one or more halogens,

(5) O-lower alkyl which may be substituted with one or more halogens,

(6) —S-lower alkyl,

(7) cyano,

(8) amino which may be substituted with one or two lower alkyls, or

(9) cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls (provided that the triazine ring to which —R⁵ is attached is attached to the nitrogen atom in the cyclic amino),

—R^(6a), —R^(6b), —R^(6c) and —R^(6d), which may be the same or different, each represent

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl, or

(6) cyano,

—W represents

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl,

(6) cyano, or

(7) a group represented by -A-B,

-A- represents

(1) —S(═O)₂—, or

(2) —C(═O)—,

—B represents

(1) lower alkyl,

(2) amino which may be substituted with one or two R^(ZA),

(3) cyclic amino (provided that -A- is attached to the nitrogen atom in the cyclic amino), or

(4) cycloalkyl,

R^(ZA) represents

(1) lower alkyl, or

(2) cycloalkyl,

—R^(1a), —R^(1b), —R^(1c) and —R^(1d), which may be the same or different, each represent

(1) —H,

(2) halogen,

(3) lower alkyl which may be substituted with one or more halogens,

(4) O-lower alkyl which may be substituted with one or more halogens,

(5) —S-lower alkyl, or

(6) cyano,

or alternatively

if —W is —H, one of —R^(1a) or —R^(1b) is a group represented by -A-B, and the other of —R^(1a) or —R^(1b), and —R^(1c) and —R^(1d), which may be the same or different, each represent any of (1) to (6) shown above,

—R² represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl which may be substituted with one or more halogens,

(5) O-lower alkyl which may be substituted with one or more halogens,

(6) —S-lower alkyl, or

(7) cyano,

—R³ and —R⁴ are as follows:

(1) one of them represents —H, and the other represents cyclic amino which may be substituted with one or more groups selected from the group consisting of R^(ZB), oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two R^(ZB) (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom in the cyclic amino),

(2) one of them represents —H, and the other represents a group represented by formula (IV) (provided that -L¹ and -L² taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring, wherein if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with R^(ZC))

(3) one of them represents —H, and the other represents a group represented by —Y-Z, or

(4) —R³ and —R⁴ taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring (provided that if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with —CO₂-(lower alkyl which may be substituted with one or more halogens)),

R^(ZB) represents

(1) lower alkyl which may be substituted with one or more groups selected from the group consisting of oxo, —OH, —O-lower alkyl, —S-lower alkyl, halogen and amino which may be substituted with one or two lower alkyls,

R^(ZC) represents

(1) lower alkyl, or

(2) —CO₂-(lower alkyl which may be substituted with phenyl),

—Y— represents

(1) piperidine-1,4-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the piperidine, and —Z is attached to the carbon atom at the 4-position of the piperidine),

(2) piperazine-1,4-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo,

(3) pyrrolidine-1,3-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the pyrrolidine, and —Z is attached to the carbon atom at the 3-position of the pyrrolidine),

(4) azetidine-1,3-diyl which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the azetidine, and —Z is attached to the carbon atom at the 3-position of the azetidine),

(5) —O—, or

(6) —N(—R^(ZD))—,

—R^(ZD) represents

(1) —H, or

(2) lower alkyl,

—Z represents

(1) cyclic amino which may be substituted with one or more groups selected from the group consisting of —R^(ZE), oxo, —OH, —O-lower alkyl, phenyl which may be substituted with halogen, piperidin-1-yl, pyrimidin-2-yl, and amino which may be substituted with one or two lower alkyls,

(2) aryl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, and cyano,

(3) cycloalkyl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, cyano, and oxo,

(4) an aromatic heterocyclic ring which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —OH, —O-lower alkyl, and cyano, or

(5) a group of formula (V)

and

—R^(ZE) represents

(1) lower alkyl which may be substituted with one or more groups selected from the group consisting of oxo, —OH, phenyl which may be substituted with halogen, and amino which may be substituted with one or two lower alkyls).

[2] The compound according to [1] or a salt thereof, wherein

—R⁵ represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl,

(5) amino which may be substituted with one or two lower alkyls, or

(6) cyclic amino (provided that the triazine ring to which —R⁵ is attached is attached to the nitrogen atom in the cyclic amino),

—R^(6a), —R^(6b), —R^(6c) and —R^(6d), which may be the same or different, each represent

(1) —H, or

(2) halogen,

—W represents

(1) —H,

(2) halogen, or

(3) a group represented by -A-B,

—R^(1a), —R^(1b), —R^(1c) and —R^(1d), which may be the same or different, each represent

(1) —H,

(2) halogen, or

(3) —O-lower alkyl,

or alternatively

if —W is —H, one of —R^(1a) or —R^(1b) is a group represented by -A-B, and the other of —R^(1a) or —R^(1b), and —R^(1c) and —R^(1d), which may be the same or different, each represent any of (1) to

(3) shown above,

—R² represents

(1) —H,

(2) —OH,

(3) halogen,

(4) lower alkyl, or

(5) —O-lower alkyl,

—R³ and —R⁴ are as follows:

(1) one of them represents —H, and the other represents cyclic amino which may be substituted with one or more groups selected from the group consisting of R^(ZB), oxo, —OH, and amino which may be substituted with one or two R^(ZB) (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom in the cyclic amino),

(2) one of them represents —H, and the other represents a group represented by formula (IV) (provided that -L¹ and -L² taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring, wherein if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with R^(ZC)),

(3) one of them represents —H, and the other represents a group represented by —Y-Z, or

(4) —R³ and —R⁴ taken together with their adjacent carbon atom represent a non-aromatic heterocyclic ring (provided that if the non-aromatic heterocyclic ring has a nitrogen atom, this nitrogen atom may be substituted with —CO₂-(lower alkyl which may be substituted with one or more halogens)),

—Y— represents

(1) piperidine-1,4-diyl (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the piperidine, and —Z is attached to the carbon atom at the 4-position of the piperidine),

(2) piperazine-1,4-diyl,

(3) pyrrolidine-1,3-diyl (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the pyrrolidine, and —Z is attached to the carbon atom at the 3-position of the pyrrolidine),

(4) azetidine-1,3-diyl (provided that the benzene ring to which —R³ or —R⁴ is attached is attached to the nitrogen atom at the 1-position of the azetidine, and —Z is attached to the carbon atom at the 3-position of the azetidine),

(5) —O—, or

(6) —N(—R^(z13))—,

—Z represents

(1) cyclic amino which may be substituted with one or more groups selected from the group consisting of —R^(ZE), oxo, —OH, phenyl which may be substituted with halogen, piperidin-1-yl, pyrimidin-2-yl, and amino which may be substituted with one or two lower alkyls,

(2) aryl,

(3) cycloalkyl,

(4) an aromatic heterocyclic ring, or

(5) a group of formula (V), and

—R^(ZE) represents (1) lower alkyl which may be substituted with one or more groups selected from the

group consisting of oxo, —OH, phenyl, and amino which may be substituted with one or two lower alkyls.

[3] A compound of formula (VI) or a salt thereof:

(wherein the symbols are as defined below:

—X¹—: a group of formula (VII) or (VIII)

—R¹⁵: —H, halogen, lower alkyl which may be substituted with one or more halogens, O-lower alkyl which may be substituted with one or more halogens, —S-lower alkyl, cyano, amino which may be substituted with one or two lower alkyls, or cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls,

—R^(16a), —R^(16b), —R^(16c) and —R^(16d), which may be the same or different: —H, halogen, lower alkyl which may be substituted with one or more halogens, O-lower alkyl which may be substituted with one or more halogens, —S-lower alkyl, or cyano,

—W¹: halogen, lower alkyl which may be substituted with one or more halogens, O-lower alkyl which may be substituted with one or more halogens, —S-lower alkyl, cyano, or a group represented by -A¹-B¹,

-A¹-: —S(═O)₂—, or —C(═O)—,

—B¹: lower alkyl, or amino which may be substituted with one or two lower alkyls,

—R^(11a), —R^(11b), —R^(11c) and —R^(11d), which may be the same or different: —H, halogen, lower alkyl which may be substituted with one or more halogens, O-lower alkyl which may be substituted with one or more halogens, —S-lower alkyl, or cyano,

—R¹²: halogen, lower alkyl which may be substituted with one or more halogens, O-lower alkyl which may be substituted with one or more halogens, —S-lower alkyl, or cyano,

—R¹³ and —R¹⁴: one of them represents —H, and the other represents cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls, or a group represented by —Y¹—Z¹,

—Y¹—: piperidine-1,4-diyl or piperazine-1,4-diyl, each of which may be substituted with one or more groups selected from the group consisting of lower alkyl and oxo, or —O— or —N(—R^(Y))—, provided that if —R¹³ or —R¹⁴ is —Y¹—Z¹ and —Y¹— is piperidine-1,4-diyl, the benzene ring to which —R¹³ or —R¹⁴ is attached is attached to the nitrogen atom at the 1-position of the piperidine, and —Z¹ is attached to the carbon atom at the 4-position of the piperidine, and wherein —R^(Y) represents —H or lower alkyl, and

—Z¹: cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls; aryl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, and cyano; cycloalkyl which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —O-lower alkyl, cyano, and oxo; or an aromatic heterocyclic ring which may be substituted with one or more groups selected from the group consisting of halogen, lower alkyl which may be substituted with one or more halogens, —OH, —O-lower alkyl, and cyano).

[4]

The compound according to [1] or a salt thereof, wherein —R^(1a), —R^(1b), —R^(1c) and —R^(1d) are each —H, —R² is —O-methyl, and —R⁴ is —H.

[5]

The compound according to [4] or a salt thereof, wherein —R³ is 4-(4-methylpiperazin-1-yl)piperidin-1-yl.

[6]

The compound according to [5] or a salt thereof, wherein —X— is a group represented by formula (II), and —R⁵ is —H.

[7]

The compound according to [6] or a salt thereof, wherein —W is a group represented by -A-B, -A- is —S(═O)₂—, and —B is isopropyl.

The compound according to [6] or a salt thereof, wherein —W is a group represented by -A-B, -A- is —S(═O)₂—, —B is amino which may be substituted with one or two R^(ZA), and R^(ZA) is methyl, ethyl, isopropyl or cyclopropyl.

The compound according to [1] or a salt thereof, wherein said compound is:

-   N-ethyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}     benzenesulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N,N-dimethylbenzenesulfonamide, -   N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[3-(4-methylpiperazin-1-yl)azetidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine, -   N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}quinazoline-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-(2-methoxy-4-piperazin-1-ylphenyl)-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-methyl-1,8-diazaspiro[4.5]decan-8-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-methyl-1,9-diazaspiro[5.5]undecan-9-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   N-cyclopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[methyl(1-methylpiperidin-4-yl)amino]phenyl}-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(4-pyrrolidin-1-ylpiperidin-1-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   1-(1-{4-[(4-{[2-(isopropylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-methoxyphenyl}piperidin-4-yl)pyrrolidin-3-ol, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(4-methylpiperazin-1-yl)phenyl]-1,3,5-triazine-2,4-diamine,     or -   N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(1-methylpiperidin-4-yl)piperazin-1-yl]phenyl}quinazoline-2,4-diamine.

The compound according to [9] or a salt thereof, wherein said compound is:

-   N-ethyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N,N-dimethylbenzenesulfonamide, -   N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[3-(4-methylpiperazin-1-yl)azetidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine, -   N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}quinazoline-2,4-diamine,     or -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine.

The compound according to [10] or a salt thereof, wherein said compound is:

-   N-ethyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N,N-dimethylbenzenesulfonamide,     or -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine.

The compound according to [11] or a salt thereof, wherein said compound is:

-   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine.

A pharmaceutical composition, which comprises the compound according to [1] or a salt thereof and a pharmaceutically acceptable excipient.

An inhibitor against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins, which comprises the compound according to [1] or a salt thereof.

A pharmaceutical composition for preventing or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer, which comprises the compound according to [1] or a salt thereof.

Use of the compound according to [1] or a salt thereof for the manufacture of a pharmaceutical composition for preventing or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer.

The compound according to [1] or a salt thereof, which is used as an active ingredient in a pharmaceutical composition for preventing or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer.

A method for preventing or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer, which comprises administering an effective amount of the compound according to [1] or a salt thereof to a patient.

The present invention will now be described in more detail below.

As used herein, the term “halogen” means F, Cl, Br or I.

The term “lower alkyl” refers to linear or branched alkyl containing 1 to 6 carbon atoms (hereinafter abbreviated as “C₁₋₆”). Examples include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, n-hexyl, and the like. Another embodiment is C₁₋₄ alkyl, and yet another embodiment is methyl, ethyl or isopropyl.

The term “cyclic amino” refers to a monovalent group of a 3- to 8-membered monocyclic non-aromatic cyclic amine which has at least one nitrogen atom and may further have the same or different one or more heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur, wherein its at least one nitrogen atom has a binding hand. Specific examples include aziridinyl, azetidinyl, pyrrolidinyl, piperidinyl, azepanyl, azocanyl, piperazinyl, homopiperazinyl, morpholinyl, oxazepanyl, thiomorpholinyl, thiazepanyl, and the like. Alternatively, another embodiment is a monovalent group of a 5- or 6-membered monocyclic non-aromatic cyclic amine. It should be noted that such a ring may be a bridged cyclic amino group, as exemplified by 2,5-diazabicyclo[2.2.1]heptane and the like, or may have an unsaturated bond in part of the ring, as exemplified by dihydropyrrolyl, tetrahydropyridyl, tetrahydropyrazyl, or the like.

The term “non-aromatic heterocyclic ring” refers to a 5- to 10-membered monocyclic non-aromatic heterocyclic ring which has 1 to 4 heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur. Examples include aziridine, azetidine, pyrrolidine, piperidine, azepane, azocane, piperazine, homopiperazine, morpholine, oxazepane, thiomorpholine, thiazepane, tetrahydropyran, tetrahydrofuran, dioxane, dioxolane, and the like. Another embodiment is a 5- or 6-membered monocyclic non-aromatic cyclic amine, including pyrrolidine, piperidine, piperazine, morpholine, thiomorpholine, and the like. It should be noted that such a ring may have an unsaturated bond in part of the ring, as exemplified by dihydropyrrole, tetrahydropyridine, tetrahydropyrazine, or the like.

The term “aryl” refers to a C₆₋₁₄ monocyclic to tricyclic aromatic hydrocarbon group, including phenyl, naphthyl, and the like. Another embodiment is phenyl.

The term “cycloalkyl” refers to an optionally bridged C₃₋₁₀ saturated cyclic hydrocarbon group, including cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, adamantyl, and the like. Other examples include those partially unsaturated, such as cyclohexenyl, cyclooctadienyl, and the like. Further examples include those in which one or two methylene groups on the ring are replaced with —O—, as exemplified by tetrahydropyranyl, tetrahydrofuranyl, dioxanyl, and the like. Still further examples include these rings which are each condensed with a benzene ring, as exemplified by indanyl, tetrahydronaphthyl, indenyl, dihydronaphthyl, dihydrochromenyl, and the like.

The term “aromatic heterocyclic ring” refers to a monovalent group of a 5- to 10-membered monocyclic or bicyclic aromatic heterocyclic ring which has 1 to 4 heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur. Examples include pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, imidazolyl, thiazolyl, oxazolyl, thienyl, furyl, indolyl, benzimidazolyl, quinolyl, isoquinolyl, quinazolinyl, quinoxalinyl, phthalazinyl, benzothiazolyl, benzoxazolyl, and the like. Another embodiment is pyridyl, pyrazinyl, pyrimidinyl, quinolyl, isoquinolyl, quinazolinyl, quinoxalinyl or phthalazinyl, and yet another embodiment is pyridyl.

The phrase “which may be substituted” is intended to include both “substituted” and “unsubstituted” embodiments. When substituted with a plurality of groups, these groups may be the same or different from each other.

The phrase “lower alkyl which may be substituted with one or more halogens” refers to, for example, lower alkyl which may be substituted with the same or different 1 to 7 halogens. Another embodiment is lower alkyl which may be substituted with 1 to 5 halogens. Yet another embodiment is lower alkyl which may be substituted with 1 to 3 halogens.

In the phrase “amino which may be substituted with one or two R^(ZA),” when this amino is substituted with two R^(ZA), these two R^(ZA) substituents may be the same or different from each other.

Some embodiments of the present invention are given below.

(1) Compounds of formula (I), wherein —R^(1a), —R^(1b), —R^(1c) and —R^(1d) are each —H. (2) Compounds of formula (I), wherein

(2-1) —R² is —O-lower alkyl, or

(2-2) —R² is —O-methyl.

(3) Compounds of formula (I), wherein

(3-1) —R³ is cyclic amino which may be substituted with lower alkyl (provided that the benzene ring to which —R³ is attached is attached to the nitrogen atom in the cyclic amino),

(3-2) —R³ is piperazinyl which may be substituted with lower alkyl (provided that the benzene ring to which —R³ is attached is attached to a nitrogen atom in the piperazine),

(3-3) —R³ is piperazinyl which may be substituted with methyl (provided that the benzene ring to which —R³ is attached is attached to a nitrogen atom in the piperazine),

(3-4) —R³ is 4-methylpiperazin-1-yl,

(3-5) —R³ is a group represented by formula (IV), in which -L¹ and -L² taken together represent cyclic amino which may be substituted with lower alkyl,

(3-6) —R³ is a group represented by formula (IV), in which -L¹ and -L² taken together represent pyrrolidine or piperidine which may be substituted with lower alkyl,

(3-7) —R³ is a group represented by formula (IV), in which -L¹ and -L² taken together represent pyrrolidine or piperidine which may be substituted with methyl,

(3-8) —R³ is a group represented by —Y-Z, in which —Y— is piperidine-1,4-diyl, piperazine-1,4-diyl, azetidine-1,3-diyl or —N(-lower alkyl)-, and —Z is cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl and —OH,

(3-9) —R³ is a group represented by —Y-Z, in which —Y— is piperidine-1,4-diyl, piperazine-1,4-diyl, azetidine-1,3-diyl or —N(-methyl)-, and —Z is piperazinyl, piperidinyl or pyrrolidinyl which may be substituted with one or more groups selected from the group consisting of methyl and —OH, or

(3-10) —R³ is 4-(4-methylpiperazin-1-yl)piperidin-1-yl.

(4) Compounds of formula (I), wherein —R⁴ is —H. (5) Compounds of formula (I), wherein

(5-1) —X— is a group represented by formula (II), and —R⁵ is —H, or

(5-2) —X— is a group represented by formula (III), and —R^(6a), —R^(6b), —R^(6c) and —R^(6c) are each —H.

(6) Compounds of formula (I), wherein

(6-1) —W is a group represented by -A-B, in which -A- is —S(═O)₂—, and —B is lower alkyl,

(6-2) —W is a group represented by -A-B, in which -A- is —S(═O)₂—, and —B is isopropyl, or

(6-3) —W is a group represented by -A-B, in which -A- is —S(═O)₂—, and —B is amino which may be substituted with one or two R^(ZA), and R^(ZA) is methyl, ethyl, isopropyl or cyclopropyl.

(7) Compounds, in which any combination of two or more of (1) to (6) shown above is applied. (8) Compounds of formula (VI), wherein —R^(11a), —R^(11b), —R^(11c) and —R^(11d) are each —H. (9) Compounds of formula (VI), wherein

(9-1) —R¹² is —O-lower alkyl, or

(2-2) —R¹² is —O-methyl.

(10) Compounds of formula (VI), wherein

(10-1) —R¹³ is cyclic amino which may be substituted with lower alkyl (provided that the benzene ring to which —R¹³ is attached is attached to the nitrogen atom in the cyclic amino),

(10-2) —R¹³ is piperazinyl which may be substituted with lower alkyl (provided that the benzene ring to which —R¹³ is attached is attached to a nitrogen atom in the piperazine),

(10-3) —R¹³ is piperazinyl which may be substituted with methyl (provided that the benzene ring to which —R¹³ is attached is attached to a nitrogen atom in the piperazine),

(10-4) —R¹³ is 4-methylpiperazin-1-yl,

(10-5) —R¹³ is a group represented by —Y¹—Z¹, in which —Y¹— is piperidine-1,4-diyl, piperazine-1,4-diyl or —N(-lower alkyl)-, and —Z¹ is cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl and —OH,

(10-6) —R¹³ is a group represented by —Y¹—Z¹, in which —Y¹— is piperidine-1,4-diyl, piperazine-1,4-diyl or —N(-methyl)-, and —Z¹ is piperazinyl, piperidinyl or pyrrolidinyl which may be substituted with one or more groups selected from the group consisting of methyl and —OH, or

(10-7) —R¹³ is 4-(4-methylpiperazin-1-yl)piperidin-1-yl.

(11) Compounds of formula (VI), wherein —R¹⁴ is —H. (12) Compounds of formula (VI), wherein

(12-1) —X¹— is a group represented by formula (VII), and —R¹⁵ is —H, or

(12-2) —X¹— is a group represented by formula (VIII), and —R^(16a), —R^(16b), —R^(16c) and —R^(16d) are each —H.

(13) Compounds of formula (VI), wherein

(13-1) —W¹ is a group represented by -A¹-B¹, in which -A¹- is —S(═O)₂—, and —B¹ is lower alkyl,

(13-2) —W¹ is a group represented by -A¹-B¹, in which -A¹- is —S(═O)₂—, and —B¹ is isopropyl, or

(13-3) —W¹ is a group represented by -A¹-B¹, in which -A¹- is —S(═O)₂—, and —B¹ is amino which may be substituted with one or two lower alkyls.

(14) Compounds, in which any combination of two or more of (8) to (13) shown above is applied.

Examples of specific compounds falling within the present invention include those selected from compound groups P, Q, R and S shown below.

Compound group P:

-   a group consisting of     N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine     and salts of this compound.

Compound group Q:

-   a group consisting of     N-ethyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide,     and -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N,N-dimethylbenzenesulfonamide,     as well as salts of these compounds.

Compound group R:

-   a group consisting of     N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   N-isopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzene     sulfonamide, -   2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[3-(4-methylpiperazin-1-yl)azetidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine,     and -   N⁴-[2-(isopropyl     sulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}quinazoline-2,4-diamine,     as well as salts of these compounds.

Compound group S:

-   a group consisting of     N-[2-(isopropylsulfonyl)phenyl]-N′-(2-methoxy-4-piperazin-1-ylphenyl)-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-methyl-1,8-diazaspiro[4.5]decan-8-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-methyl-1,9-diazaspiro[5.5]undecan-9-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   N-cyclopropyl-2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}benzenesulfonamide, -   N-[2-(isopropylsulfonyl)phenyl]-N′-{2-methoxy-4-[methyl(1-methylpiperidin-4-yl)amino]phenyl}-1,3,5-triazine-2,4-diamine, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(4-pyrrolidin-1-ylpiperidin-1-yl)phenyl]-1,3,5-triazine-2,4-diamine, -   1-(1-{4-[(4-[2-(isopropylsulfonyl)phenyl]amino-1,3,5-triazin-2-yl)amino]-3-methoxyphenyl}piperidin-4-yl)pyrrolidin-3-ol, -   N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(4-methylpiperazin-1-yl)phenyl]-1,3,5-triazine-2,4-diamine,     and -   N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(1-methylpiperidin-4-yl)piperazin-1-yl]phenyl}quinazoline-2,4-diamine,     as well as salts of these compounds.

The compounds of formula (I) may have tautomers and/or geometrical isomers, depending on the type of their substituents. Even when the compounds of formula (I) appear herein only in one isomer form, the present invention encompasses the other isomers, and also encompasses separated isomers or mixtures thereof.

Further, since some compounds of formula (I) have an asymmetric carbon atom or axial asymmetry, optical isomers based on this asymmetry may also exist. The present invention also encompasses separated optical isomers of the compounds of formula (I) or mixtures thereof.

Furthermore, the present invention encompasses pharmaceutically acceptable prodrugs of the compounds represented by formula (I). The term “pharmaceutically acceptable prodrug” refers to a compound having a group which can be converted into an amino group, a hydroxyl group, a carboxyl group or the like by solvolysis or under physiological conditions. Examples of a prodrug-forming group include those described in Prog. Med., 5, 2157-2161 (1985) or those described in “Development of Pharmaceuticals” (Hirokawa Publishing, 1990) vol. 7, Molecular Design 163-198.

Likewise, salts of the compounds of formula (I) are pharmaceutically acceptable salts of the compounds of formula (I). The compounds of formula (I) may form acid or base addition salts, depending on the type of their substituents. Specific examples include acid addition salts with inorganic acids (e.g., hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, nitric acid, phosphoric acid, and the like) or with organic acids (e.g., formic acid, acetic acid, propionic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, maleic acid, lactic acid, malic acid, mandelic acid, tartaric acid, dibenzoyltartaric acid, ditoluoyltartaric acid, citric acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, aspartic acid, glutamic acid, and the like), salts with inorganic bases (e.g., sodium, potassium, magnesium, calcium, aluminum, and the like) or with organic bases (e.g., methylamine, ethylamine, ethanolamine, lysine, ornithine, and the like), salts with various amino acids and amino acid derivatives (e.g., acetylleucine, and the like), as well as ammonium salt, etc.

Moreover, the present invention also encompasses the compounds of formula (I) and salts thereof in the form of various hydrates, solvates, and crystalline polymorphic substances. The present invention also encompasses the compounds labeled with various radioactive or non-radioactive isotopes.

The compounds of formula (I) and pharmaceutically acceptable salts thereof can be prepared by applying various known synthesis methods on the basis of characteristics derived from their skeletal structure or the type of their substituents. In some cases, depending on the type of functional group, it is technically effective to replace such a functional group with an appropriate protecting group (a group which can be easily converted into the original functional group) at the starting material stage or at the intermediate stage. Examples of such a protecting group include those described in Greene and Wuts, “Protective Groups in Organic Synthesis (third edition, 1999)” and so on, which may be selected and used as appropriate, depending on reaction conditions. In such a method, after introduction of the protecting group and subsequent reaction, the protecting group may be removed if necessary to obtain a desired compound.

Likewise, a prodrug of the compound of formula (I) can be prepared by introducing a specific group at the starting material stage or at the intermediate stage, as in the case of the above protecting group, or by subjecting the obtained compound of formula (I) to further reaction. The reaction may be accomplished by applying conventional esterification, amidation, dehydration or other techniques known to those skilled in the art.

Explanation will be given below of typical processes for preparing the compounds of formula (I). Each process may also be accomplished by reference to the documents cited in this explanation. It should be noted that the processes of the present invention are not limited to the examples illustrated below.

(Preparation Process 1)

(wherein -L represents a leaving group (the same applying hereinafter))

This process is intended to prepare the compound (1) of the present invention by reacting compound (1a) having a leaving group with aniline derivative (1b). Examples of a leaving group used for this purpose include halogen (e.g., F, Cl, and the like), sulfonyloxy (e.g., methanesulfonyloxy, p-toluenesulfonyloxy, trifluoromethanesulfonyloxy, and the like), as well as lower alkylsulfanyl or lower alkanesulfonyl.

In this reaction, compound (1a) having a leaving group and aniline derivative (1b) are used in equal amounts or one of them is used in an excessive amount. A mixture of these compounds is stirred in a solvent inert to the reaction or in the absence of a solvent under cooling to reflux conditions, preferably at 0° C. to 80° C., generally for 0.1 hours to 5 days. Examples of a solvent used for this purpose include, but are not particularly limited to, aromatic hydrocarbons (e.g., benzene, toluene, xylene, and the like), ethers (e.g., diethyl ether, tetrahydrofuran, dioxane, dimethoxyethane, and the like), halogenated hydrocarbons (e.g., dichloromethane, 1,2-dichloroethane, chloroform, and the like), alcohols (e.g., methanol, ethanol, 2-propanol, and the like), N,N-dimethylformamide, dimethyl sulfoxide, ethyl acetate, acetonitrile, and mixtures thereof. The reaction may be performed in the presence of an organic base (e.g., triethylamine, N,N-diisopropylethylamine, N-methylmorpholine, or the like) or an inorganic base (e.g., potassium carbonate, sodium carbonate, potassium hydroxide, or the like), because it is advantageous for smooth reaction in some cases.

When the reaction is performed in the presence of such a base as shown above, depending on the properties or the like of starting compounds, the desired reaction is impossible or difficult to proceed, for example, due to decomposition or the like of the starting compounds. In this case, the reaction may be performed in the presence of a mineral acid (e.g., hydrochloric acid, hydrobromic acid, and the like), an organic acid (e.g., acetic acid, propionic acid, and the like) or a sulfonic acid (e.g., methanesulfonic acid, p-toluenesulfonic acid, and the like), because it is advantageous for smooth reaction in some cases.

DOCUMENTS

-   S. R. Sandler and W. Karo, “Organic Functional Group Preparations,”     second edition, vol. 1, Academic Press Inc., 1991 -   The Chemical Society of Japan, “Fifth Series of Experimental     Chemistry,” vol. 14 (2005) (MARUZEN Co., Ltd., Japan)

(Preparation Process 2)

This process is intended to prepare the compound (1) of the present invention by reacting compound (2a) having a leaving group with aniline derivative (2b).

In this reaction, the procedure of Preparation Process 1 may be applied.

(Starting Material Synthesis)

(wherein L₁ and L₂ each represent a leaving group selected from the members of L shown above (the same applying hereinafter))

This process is intended to prepare compound (1a) by reacting compound (3) having leaving groups with aniline derivative (2b).

In this reaction, the procedure of Preparation Process 1 may be applied.

This process is intended to prepare compound (2a) by reacting compound (3) having leaving groups with aniline derivative (1b).

In this reaction, the procedure of Preparation Process 1 may be applied.

The compound of formula (I) is isolated and purified as a free compound or as a pharmaceutically acceptable salt, hydrate, solvate or crystalline polymorphic substance thereof. A pharmaceutically acceptable salt of the compound of formula (I) may also be prepared by being subjected to conventional salt-forming reaction.

Isolation and purification may be accomplished by applying conventional chemical operations such as extraction, fractional crystallization, various types of fractionation chromatography, etc.

Various isomers can be prepared by selecting appropriate starting compounds or can be separated on the basis of differences in the physical and chemical properties of isomers. For example, optical isomers can be derived into optically pure isomers by conventional optical resolution techniques (e.g., fractional crystallization resulting in a diastereomer salt with an optically active base or acid, chromatography on a chiral column or the like, and the like). They can also be prepared from appropriate optically active starting compounds.

The compounds of formula (I) were confirmed for their pharmacological activity in the following tests. Unless otherwise specified, the test examples shown below may be accomplished in a known manner and, when using commercially available reagents, kits, or the like, may be accomplished in accordance with the instructions attached to these commercially available products.

Test Example 1 Evaluation of Inhibitory Activity Against the Kinase Activity of EML4-ALK Fusion Protein v1

EML4-ALK fusion protein v1 (purified from BA/F3 cells expressing EML4-ALK fusion protein v1) was investigated for its phosphorylation activity toward a peptide substrate by using a kinase activity detection kit (HTRF KinEASE-TK; Cisbio Inc.). Test compounds were each added to a reaction solution containing the enzyme protein to give 8 final concentrations from 1000 nM to 0.3 nM (100 nM to 0.03 nM for TAE684), followed by addition of ATP and reaction for 1 hour. The ATP concentration used was 100 μM. Another reaction solution was prepared to contain the enzyme protein but no test compound (in which the solvent DMSO alone was added at 0.4% in place of the test compound), followed by reaction in the same manner with or without ATP addition. In the absence of the test compound, the phosphorylation count without ATP addition and with ATP addition was assumed to be 100% inhibition and 0% inhibition, respectively. The concentration causing 50% inhibition (IC₅₀) was calculated for each test compound by the logistic regression method.

As a result, the compounds of the present invention and TAE684 were found to have inhibitory activity against the kinase activity of EML4-ALK fusion protein v1. Table 1 shows the IC₅₀ values obtained for some compounds of the present invention and TAE684. Ex denotes Example No.

TABLE 1 Ex IC₅₀(nM) Ex IC₅₀(nM)  1 42 128 2.3  23 17  12 61  24 29 149 40  45 66 166 49  52 72  7 50  58 25 171 33  63 26 176 150  72 51 TAE684 0.63 120 74 123 33

Test Example 2 Evaluation of Inhibitory Activity Against the Kinase Activity of Mutant EGFR (L858R) Protein

Mutant EGFR (L858R) protein (Cana Biosciences Inc., Japan) was investigated for its phosphorylation activity toward a peptide substrate by using a kinase activity detection kit (HTRF KinEASE-TK; Cisbio Inc.). Test compounds were each added to a reaction solution containing the enzyme protein to give 8 final concentrations from 10000 nM to 3 nM, followed by addition of ATP and reaction for 1 hour. The ATP concentration used was 5 μM. Another reaction solution was prepared to contain the enzyme protein but no test compound (in which the solvent DMSO alone was added at 0.4% in place of the test compound), followed by reaction in the same manner with or without ATP addition. In the absence of the test compound, the phosphorylation count without ATP addition and with ATP addition was assumed to be 100% inhibition and 0% inhibition, respectively. The concentration causing 50% inhibition (IC₅₀) was calculated for each test compound by the logistic regression method.

As a result, the compounds of the present invention and TAE684 were found to have inhibitory activity against the kinase activity of mutant EGFR (L858R) protein. Table 2 shows the IC₅₀ values obtained for some compounds of the present invention and TAE684. Ex denotes Example No.

TABLE2 Ex IC₅₀(nM)  23 120 123 100 128  98 TAE684  92

Test Example 3 Evaluation of Inhibitory Effect Against Anchorage-Independent Cell Growth of Human Non-Small Cell Lung Cancer Cell Line NCI-H2228 Cells (EML4-ALK Fusion Protein-Expressing Cells)

Measurement for anchorage-independent cell growth (colony method, etc) has been known to be a system for investigating the anticancer action (pharmacological effect) of test compounds (Clinical Oncology, second edition, Cancer and Chemotherapy Publishers Inc.). In place of the colony method, there is a following method using spheroid plates for measuring the growth of non-attached cells.

In a 96-well spheroid plate (Sumilon Celltight Spheroid 96U; Sumitomo Bakelite Co., Ltd., Japan), human non-small cell lung cancer cell line NCI-H2228 cells were seeded at 2000 cells per well in RPMI1640 medium (Invitrogen) containing 10% fetal bovine serum. NCI-H2228 cells are those expressing another EML4-ALK fusion protein, which is different from the EML4-ALK fusion protein v1 because it is encoded by an EML4-ALK fusion polynucleotide whose fusion point on EML4 cDNA is different from that of the EML4-ALK fusion polynucleotide v1, but whose ALK region is the same as that of the EML4-ALK fusion polynucleotide v1. The above NCI-H2228 cells seeded in the plate were cultured overnight under 5% CO₂ at 37° C., followed by addition of a test compound (final concentration: 10 μM to 1 nM). As a negative control, DMSO used as a solvent was added at the same concentration as the test compound. Then, the cells were cultured under 5% CO₂ at 37° C. for 5 days. A cell counting reagent (CellTiter-Glo™ Luminescent Cell Viability Assay; Promega) was added and agitated for 20 minutes, followed by measurement with a luminometer (ML3000 microtiter plate luminometer; Dynatech Laboratories). Assuming that the value measured for the medium alone and the value measured for the negative control were 100% inhibition and 0% inhibition, respectively, the inhibition rate was calculated for each compound to thereby determine the concentration causing 50% inhibition (IC₅₀ value) by the logistic regression method.

As a result, the compounds of the present invention and TAE684 were found to have growth inhibitory activity against human non-small cell lung cancer cell line NCI-H2228 cells. Table 3 shows the IC₅₀ values obtained for some compounds of the present invention and TAE684. Ex denotes Example No.

TABLE 3 Ex IC₅₀(nM) Ex IC₅₀(nM)  1  473 128 64  23  71  12 134  24  125 149 62  45 1039 166 125  52  159  7 87  58  156 171 61  63  96 176 119  72  93 TAE684 8.5 120  168 123  30

Test Example 4 Evaluation of Inhibitory Effect Against Anchorage-Independent Cell Growth of Human Non-Small Cell Lung Cancer Cell Line HCC827 Cells (Mutant EGFR (with Partial Deletion of Exon 19 in EGFR) Protein-Expressing Cells, American Type Culture Collection)

Evaluation was performed in the same manner as shown in Test Example 3.

As a result, the compounds of the present invention and TAE684 were found to have growth inhibitory activity against human non-small cell lung cancer cell line HCC827 cells. Table 4 shows the IC₅₀ values obtained for some compounds of the present invention and TAE684. Ex denotes Example No.

TABLE 4 Ex IC₅₀(nM) 1 2513 23 272 24 1027 45 1899 52 820 58 648 63 791 72 670 120 660 123 238 128 175 12 509 149 512 166 419 7 214 171 252 176 496 TAE684 301

From the results of Test Examples 1 to 4 shown above, it was confirmed that the compounds of the present invention and TAE684 had inhibitory activity against the kinase activity of EML4-ALK fusion protein v1 and growth inhibitory activity against human non-small cell lung cancer cell line NCI-H2228 cells, and that TAE684 had stronger activity than the compounds of the present invention. It was also confirmed that the compounds of the present invention and TAE684 had inhibitory activity against the kinase activity of mutant EGFR (L858R) protein and growth inhibitory activity against human non-small cell lung cancer cell line HCC827 cells, and that the compounds of the present invention and TAE684 had almost equal activity.

Test Example 5 Toxicity Test in Rats

Test compounds were each suspended in a 0.5% aqueous methylcellulose solution and repeatedly administered to SD rats (two females and four males in each group) by the oral route at each dose for 7 days. TAE684 was administered at 3, 10, 30 and 100 mg/kg, while the compound of Example 23 was administered at 10, 30, 100 and 300 mg/kg.

The results obtained are shown in Table 5.

TABLE 5 Dose Compound of Example 23 TAE684 Male Female Male Female (4 rats) (2 rats) (4 rats) (2 rats) Non-toxic dose  10  10  3  3 Bone marrow 100 100  10  10 inhibition Exacerbation of (>300) (>300) 100  30 common symptoms Moribund condition (>300) (>300) (>100) 100

At the doses used in this test, TAE684 produced the following clear toxic symptoms: exacerbation of common symptoms (e.g., decreased autonomic movement, eyelid closure, skinniness, blepharophimosis) in the females of the 30 mg/kg group; these findings as well as prone position, bradypnea and hypersalivation in the females and males of the 100 mg/kg group; and remarkable exacerbation of the post-administration state at Day 7 in the two females (all cases) of the 100 mg/kg group (for this reason, these two cases were examined by moribund autopsy). In contrast, although the compound of Example 23 was found to cause a decrease in the amount of feces in 2 of the 4 males in the 300 mg/kg group, there was no case in each dose group, which showed exacerbation of common symptoms during administration for 7 days. Also, no moribund case was observed in each dose group.

Namely, the compound of Example 23 has an effect equal to TAE684 on growth inhibition of mutant EGFR protein-expressing cells, but on the other hand causes no exacerbation of common symptoms or shows no moribund case even when administered at a dose of 300 mg/kg, which is higher than the dose of 30 mg/kg or 100 mg/kg at which exacerbation of common symptoms or moribund cases are observed for TAE684. Thus, the compound of Example 23 is regarded as a safer compound than TAE684.

Based on the above results, in cancer therapy for EML4-ALK fusion polynucleotide-positive cancer patients, TAE684 has fears about safety (e.g., exacerbation of common symptoms) at a lower dose than in the compound of Example 23 (Test Example 5), whereas TAE684 appears to produce a therapeutic effect at a lower dose than in the compound of Example 23 (Test Examples 1 and 3). It is therefore inferred that the compound of Example 23 and TAE684 are almost comparable to each other in terms of a balance between therapeutic effect and safety. On the other hand, in cancer therapy for mutant EGFR polynucleotide-positive cancer patients, the compound of Example 23 and TAE684 appear to produce a therapeutic effect at almost the same dose (Test Examples 2 and 4), whereas TAE684 has fears about safety (e.g., exacerbation of common symptoms) at a lower dose than in the compound of Example 23 (Test Example 5). It is therefore concluded that the compound of Example 23 is superior to TAE684 in terms of a balance between therapeutic effect and safety.

Thus, even if the compound of Example 23 and TAE684 can both produce a therapeutic effect on cancer with some degree of safety during cancer therapy for EML4-ALK fusion polynucleotide-positive cancer patients, the so-called margin of safety is narrower in TAE684 than in the compound of Example 23 in cancer therapy for mutant EGFR polynucleotide-positive cancer patients, and hence TAE684 has a possibility of failing to provide a sufficient therapeutic effect when the dose should be reduced to ensure safety. In contrast, the compound of Example 23 has a wider margin of safety than TAE684 and hence can be expected to be administered at a dose which ensures a sufficient therapeutic effect. Namely, the compound of Example 23 is expected as a therapeutic agent for cancer that is applicable to a wider spectrum of cancer patients than in TAE684.

Test Example 6 Kinase Inhibition Profiling

The inhibition rates against 88 types of kinases (ABL, ACK, AXL, BMX, BTK, CSK, DDR2, EGFR, EphA2, EphB4, FES, FGFR1, FGFR3, FLT1, FLT4, FMS, INSR, JAK2, JAK3, KDR, MER, MUSK, PDGFRa, RET, TEC, TIE2, TYK2, TYRO3, ABL[T315I], EGFR[L858R], EGFR[T790M], AKT2, AurC, BMPR1A, BRAF, BRAF[V600E], CaMK2a, CaMK4, CDK3, CHK2, CK1a, CK1d, COT, CRIK, DAPK1, DLK, Erk5, GSK3a, GSK3b, IKKa, IKKb, IKKe, IRAK4, JNK1, JNK3, MAP2K2, MAP2k3, MAP2K4, MAP2K5, MAP2K7, MAP3K1, MAP3K2, MAP3K3, MAP3K4, MAP3K5, MAPKAPK2, MAPKAPK3, MAPKAPK5, MLK1, MLK2, MLK3, MNK1, MNK2, MSK1, NEK2, p38d, p38g, PAK6, PHKG1, PIM1, PKACa, PKCh, PKD2, ROCK1, RSK2, SRPK1, TAK1, TTK) were calculated for each test compound at 100 nM. Activity measurement was made by Carna Biosciences Inc., Japan, and the data were analyzed as follows: assuming that the average signal of control wells containing all reaction components was 0% inhibition and the average signal in the absence of the enzyme was 100% inhibition, the inhibition rate was calculated for each test substance from the average signal of two test wells.

As a result, at a concentration of 100 nM, TAE684 showed 50% or more inhibitory activity against 29 types of kinases, whereas the compound of Example 23 showed inhibitory activity only against 4 types.

Namely, TAE684 has strong inhibitory activity against a wide range of kinases, whereas the compound of Example 23 at the same concentration has a different inhibition profile than that of TAE684 and appears to be highly selective for specific kinases, i.e., appears to have much fewer fears about safety than TAE684, which fears are induced by inhibition of non-target kinases responsible for side effects.

In addition, when a careful examination was actually made on various kinase inhibition profiles, kinases against which TAE684 has higher inhibitory activity than the compound of Example 23 were MUSK, MER and PHKG1. TAE684 showed 90% or more inhibitory activity against these kinases at concentration of 100 nM, whereas the compound of Example 23 showed little inhibitory activity at the same concentration (less than 20%).

MUSK is a kinase essential for acetylcholine receptor functions in the neuromuscular junction. If people have a mutation in this kinase or are positive for anti-MUSK antibody, they are known to develop a hereditary disease with myasthenia showing symptoms such as blepharoptosia, hypersalivation, and respiratory disturbance (Hum Mol Genet. 2004 13, 3229-3240 and Nat. Med. 2001 7, 365-368). There are many symptoms in common between exacerbation of common symptoms observed for TAE684 in Test Example 5 and phenotypes caused by mutations in MUSK. Thus, exacerbation of common symptoms observed for TAE684 administered at 30 mg/kg or more may have some relationship with MUSK inhibition.

MER is a kinase required for retinal cells to maintain their survival. If people have a mutation in this kinase, they are known to develop a hereditary disease with retinitis pigmentosa responsible for gradual narrowing of the visual field, which may lead to blindness (Nature Genet. 2000 26, 270-271). Thus, the possibility of TAE684 to cause a defect in retinal cells due to its inhibitory activity against MER cannot be denied. In contrast, the compound of Example 23 appears to have almost no fear of causing a defect in retinal cells, because its inhibitory activity against MER is weaker than that of TAE684.

PHKG1 is an enzyme essential for glycogen metabolism in muscle, and is known to contribute to a hereditary disease caused by mutations in enzyme complex subunits, which is feared to show glycogenosis, muscle ache during exercise, easy fatigue, myotonia, liver swelling, abdominal swelling, glycogenosis (glycogen accumulation)-induced muscle tissue atrophy, and metabolic myopathy (Am. J. Med. Genet. 2005 133A, 82-84). Thus, the possibility of TAE684 to cause a defect in muscle tissue due to its inhibitory activity against PHKG1 cannot be denied. In contrast, the compound of Example 23 appears to have almost no fear of causing a defect in muscle tissue, because its inhibitory activity against PHKG1 is weaker than that of TAE684.

On the other hand, kinases against which the compound of Example 23 has higher inhibitory activity than TAE684 are MNK1 and MNK2. TAE684 showed 4.8% and 32% inhibitory activity against these kinases, respectively, at 100 nM, whereas the compound of Example 23 showed 60% and 80% inhibitory activity at the same concentration. However, it has been reported that mice whose MNK1 and MNK2 genes are both disrupted will grow normally (Molecular and Celluar Biology 2004 24, 6539-6549). It is therefore difficult to believe that serious diseases are caused by the inhibitory activity of the compound of Example 23 against MNK1 and MNK2.

Test Example 7 Evaluation of Inhibitory Activity Against the Kinase Activity of MUSK Protein

MUSK protein (Carna Biosciences Inc., Japan) was investigated for its phosphorylation activity toward a peptide substrate by using a kinase activity detection kit (HTRF KinEASE-TK; Cisbio Inc.). Test compounds were each added to a reaction solution containing the enzyme protein to give 8 final concentrations from 10000 nM to 3 nM, followed by addition of ATP and reaction for 1 hour. The ATP concentration used was 10 μM. Another reaction solution was prepared to contain the enzyme protein but no test compound (in which the solvent DMSO alone was added at 0.4% in place of the test compound), followed by reaction in the same manner with or without ATP addition. In the absence of the test compound, the phosphorylation count without ATP addition and with ATP addition was assumed to be 100% inhibition and 0% inhibition, respectively. The concentration causing 50% inhibition (IC₅₀) was calculated for each test compound by the logistic regression method.

As a result, the compounds of the present invention and TAE684 were found to have inhibitory activity against the kinase activity of MUSK protein. Table 6 shows the IC₅₀ values obtained for some compounds of the present invention and TAE684. Ex denotes Example No.

TABLE 6 Ex IC₅₀(nM)  23 1500 123 1100 128 1800 TAE684  17

From the results of Test Example 7 shown above, it was confirmed that TAE684 had very strong inhibitory activity against the kinase activity of MUSK protein, when compared to the compounds of the present invention. There are many symptoms in common between exacerbation of common symptoms observed for TAE684 in Test Example 5 and phenotypes caused by mutations in MUSK. Thus, exacerbation of common symptoms observed for TAE684 administered at 30 mg/kg or more may have some relationship with MUSK inhibition.

Test Example 8 Antitumor Test (In Vivo) on NCI-H2228 Cells

3×10⁶ cells of NCI-H2228 cells suspended in PBS were inoculated subcutaneously by injection to the back of 5 weeks old male NOD/SCID mice (Charles River Japan, Inc.). After 3 weeks of the inoculation, the administration of test compounds was initiated. The test was conducted in the solvent group and test compound groups, 6 animals per group. The test compounds were each dissolved in a solvent composed of 10% 1-methyl-2-pyrrolidinone (SIGMA-ALDRICH Inc.)/90% polyethylene glycol 300 (Fluka Inc.) and administered orally at a dose of 3 mg/kg. Administrations were performed once a day for 14 days, and body weight and tumor size were measured every other day. Tumor volume was calculated using the following formula.

[Tumor volume (mm³)]=[Tumor major axis (mm)]×[tumor minor axis (mm)]²×0.5

Assuming that the tumor volume of the solvent group on the day of starting and the day of finishing administration was 100% inhibition and 0% inhibition, respectively, the inhibition rate was calculated for each compound.

As a result, the compounds of the present invention were found to have an antitumor effect on NCI-H2228 cells (tumor). Among them, the compounds of Examples 23 and 123 inhibited the growth of NCI-H2228 cells (tumor) by 116% and 108%, respectively.

Thus, when orally administered, the compounds of the present invention inhibited tumor growth in mice inoculated with H2228 cells, thereby confirming that the compounds of the present invention had oral activity.

In view of the foregoing, in Test Examples 1 to 4, the compounds of the present invention were confirmed to have inhibitory activity against the kinase activity of both EML4-ALK fusion protein v1 and mutant EGFR (L858R) protein, as well as growth inhibitory activity against human non-small cell lung cancer cell lines NCI-H2228 and HCC827. In Test Example 8, the compounds of the present invention were also confirmed to have an antitumor effect on NCI-H2228 cells (tumor) based on the above actions. Further, in Test Example 5, the compounds of the present invention were confirmed to be safer than TAE684, showing no toxicity even when administered at a dose of 300 mg/kg which is higher than the dose at which exacerbation of common symptoms was observed in TAE684. These indicate that the compounds of the present invention are useful as active ingredients in pharmaceutical compositions for preventing and/or treating cancer, such as lung cancer in one embodiment, non-small cell lung cancer or small cell lung cancer in another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer in yet another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer in yet another embodiment, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer in yet another embodiment.

In Non-patent Document 7, it has been confirmed that among EML4-ALK fusion protein-expressing lung cancer cell lines, there are some lung cancer cell lines expressing a constitutively activated EGFR protein together with the EML4-ALK fusion protein. To inhibit the growth of these lung cancer cell lines, both proteins should be inhibited (Non-patent Document 7). The compounds of the present invention have equal inhibitory activity against both EML4-ALK fusion protein v1 and mutant EGFR (L858R) protein, and hence can be expected to have excellent growth inhibitory activity against such lung cancer cell lines at a certain dose. Thus, the compounds of the present invention would be useful as active ingredients in pharmaceutical compositions for preventing and/or treating EML4-ALK fusion polynucleotide-positive and mutant EGFR polynucleotide-positive cancer. Moreover, the compounds of the present invention can be used at a single dose for both EML4-ALK fusion polynucleotide-positive cancer and mutant EGFR polynucleotide-positive cancer.

In contrast, although TAE684 has inhibitory activity against the kinase activity of mutant EGFR (L858R) protein and growth inhibitory activity against HCC827 cells, each activity being equal to that of the compounds of the present invention, TAE684 started to develop serious toxicity at a lower dose than the compound of Example 23 in Test Example 5. Thus, when compared to the compound of Example 23, TAE684 has fears about safety in its effective dose required to produce a sufficient growth inhibitory effect on mutant EGFR polynucleotide-positive cancer.

The compounds of formula (I) were also confirmed for their pharmacological activity in the following series of tests. Unless otherwise specified, the test examples shown below may be accomplished in a known manner and, when using commercially available reagents and/or kits, may be accomplished in accordance with the instructions attached to these commercially available products.

The full-length ALK cDNA was kindly provided by Dr. Steve Morris, St. Jude Children's Research Hospital. This research project was approved by the ethical review committee for gene analysis research of Jichi Medical University.

The anti-phosphorylated ALK antibody used was a product of Cell Signaling Technology Inc., and the anti-ALK antibody used was a product of NEOMARKERS Inc.

Test Example 9 Isolation of EML4-ALK Fusion Polynucleotide v1

(1) Construction of cDNA Library

Using a RNA purification kit (RNeasy Mini Column; Qiagen Inc.), RNA was extracted from a resected specimen of lung adenocarcinoma of a 62 year old male who gave informed consent and cDNA was synthesized using reverse transcriptase (Power Script Reverse Transcriptase) and primers (an oligonucleotide of SEQ ID NO: 3 and CDS primer IIA) (all from Clontech Inc.). After selectively amplifying the full-length cDNA by polymerase chain reaction (PCR) (17 cycles of 98° C. for 10 seconds and 68° C. for 6 minutes) using a primer (5′-PCR primer IIA; Clontech Inc.) and a polymerase (primeSTAR HSDNA polymerase, Takara Bio Inc.), a BstX1 adapter (Invitrogen Inc.) was attached to the both ends of cDNA. The cDNA thus obtained was ligated to a retrovirus plasmid, and a retrovirus plasmid library was constructed by introducing this plasmid to E. coli DH10B (Invitrogen Inc.). As a result, the plasmid library containing clones more than 1,500,000 colony forming units in total has been successfully constructed.

(2) Focus Formation Assay

2 μg of the plasmid of the library described above and 0.5 μg of a plasmid for packaging (pGP and pE-eco, both of which were obtained from Takara Bio Inc.) were transfected into BOSC23 packaging cells using a transfection reagent. Two days after the transfection, the culture supernatant was recovered as a solution of recombinant retrovirus library, mixed with polybrene (Sigama Inc.) at a concentration of 4 μg/ml, and the mixture was added to mouse 3T3 cells at MOI (multiplicity of infection) of 0.1 concentration. Two days later, the culture supernatant of 3T3 cells was changed to DMEM-F12 medium (Invitrogen Inc.) supplemented with 5% bovine serum (Invitrogen Inc.) and 2 mM L-glutamine, and the cells were cultured 2 more weeks to obtain 10 or more kinds of transformed foci. After isolating each 3T3 cell clone, the culturing of the clones was continued separately, and the genomic DNA of each clone was extracted. The viral cDNA integrated in each 3T3 clone was amplified and recovered by carrying out PCR (30 cycles of 98° C. for 10 seconds and 68° C. for 6 minutes) using 10 ng of the genomic DNA as a template, 5′-PCR primer IIA primer and DNA polymerase (PrimeStar HS DNA polynerase; Takara Bio Inc.), and cloned into pT7Blue-2 vector.

One of the cDNA thus obtained was 3926 base pair long (SEQ ID NO: 1) and had a single long open reading frame (from the 271st to 3447th nucleotides of SEQ ID NO: 1) coding for a protein having 1059 amino acid residues (SEQ ID NO: 2). Interestingly, about half of the amino-terminus (1-496 amino acid residues of SEQ ID NO: 2) of a protein encoded by this cDNA having a novel full-length sequence was perfectly matched to 1-496 amino acid residues of echinoderm microtubule associated protein like-4 (EML4, GenBank accession No. NM_(—)019063), and on the other hand, about half of the carboxyl terminus (497-1059 amino acid residues of SEQ ID NO: 2) was perfectly matched to the amino acid sequence of anaplastic lymphoma kinase (ALK, GenBank accession No. AB209477). From the above results, the present cDNA was believed to be a fused cDNA between EML4 cDNA and ALK cDNA. Further, the obtained cDNA (cDNA for EML4-ALK fusion polynucleotide v1) contained a domain of ALK tyrosine kinase.

Test Example 10 Detection of EML4-ALK Fusion Polynucleotide in Clinical Specimens

cDNAs were synthesized from 33 cases of clinical specimens (resected specimens of non-small cell lung cancer) and from peripheral monocytes of one case of a normal healthy subject.

To detect the cDNA of EML4-ALK fusion polynucleotide v1, PCR (50 cycles of 94° C. for 15 seconds, 60° C. for 30 seconds and 72° C. for 1 minute) was carried out using a quantitative PCR kit (QuantiTect SYBR Green; Qiagen Inc.), the cDNAs as substrates prepared from the clinical specimens and the normal healthy subject described above and oligonucleotides of SEQ ID NOs: 4 and 5 as primers. Using the same specimens, PCR amplifications of the glyceraldehyde-3-phosphate dehydrogenase (hereinafter GAPDH) cDNA was tried as a control. To detect the GAPDH cDNA, oligonucleotides consisting of the nucleotide sequences represented by SEQ ID NOs: 6 and 7 were used as primers. Amplified respective samples were electrophoresed with a size marker DNA (Marker: 50 bp ladder, Invitrogen Inc.). As a result, as shown in the upper part of FIG. 1, in the 3 cases, the cDNA of EML4-ALK fusion polynucleotide v1 was detected. Further, in all the cases analyzed, an amplification of the GAPDH cDNA was confirmed clearly (Lower part of FIG. 1). In addition, the nucleotide sequences of the PCR products identified in these 3 cases were analyzed and the result confirmed that all had the same sequence (the 247 bp including the fusion point of the EML4 gene and the ALK gene; SEQ ID NO: 8). That is, the result of the analyses of the 33 cases of non-small cell lung cancer confirmed that the fusion of the EML4 gene and the ALK gene occurs in 9.1% of the cases (3/33 cases).

Mutation in the EGFR gene has been known to be one of the causes of lung cancer. In the 33 specimens of the cases analyzed as described above, the analysis of the presence of an abnormality in the nucleotide sequence of the EGFR gene according to the known method confirmed a partial deletion of exon 19 in 6 cases. The cases having the EGFR gene mutation and the cases positive for the EML4-ALK fusion polynucleotide belonged to different subgroups. That is, the existing therapeutic agents, which show a therapeutic effect on lung cancer patients having the EGFR gene mutation, are expected to be not effective for the lung cancer patients who are positive for the EML4-ALK fusion polynucleotide.

Also, the 33 cases analyzed as described above were subjected to the investigation whether the full-length ALK gene existed, and it was found that it existed in 8 cases. The specimens of the 7 cases among these 8 cases did not contain the EML4-ALK fusion polynucleotide. That is, the full-length ALK gene did not exist in the 2 cases among the 3 cases where the EML4-ALK fusion polynucleotide was positive.

Test Example 11 Investigation of Tumorgenicity of EML4-ALK Fusion Polypeptide v1

EML4-ALK (K589M)/pMXS, in which the 589th amino acid (ATP binding site), a lysine residue, of the EML4-ALK fusion polypeptide v1 was replaced with methionine, was produced using EML4-ALKv1/pMXS (prepared from a clone in which the EML4-ALK fusion polynucleotide v1 was cloned in the forward orientation into pT7Blue-2 vector, which clone was further digested with restriction enzymes EcoRI and SalI to release the insert, which was then subcloned into the EcoRI-SalI site of pMXS (J. Biol. Chem., vol. 275, p. 24945-24952, 2000)) as a substrate and using a mutation introducing kit (QuickChange Site-Directed Mutagenesis Kit; Stratagene Inc.). In the reaction, oligonucleotides of SEQ ID NO: 9 and SEQ ID NO: 10 were used. The ALK cDNA (Morris, S W et al, Science. 1994 Mar. 4; 263 (5151):1281-4) was cloned into a retrovirus vector pMXS according to the standard method (designated as ALK/pMXS and ALK/pMX-iresCD8, respectively).

EML4-ALKv1/pMXS described above, full-length ALK/pMXS, a plasmid expressing EML4-ALK (K589M)/pMXS and a blank vector without inserted cDNA (pMXS) were transfected into 3T3 fibroblast cells by the phosphate calcium method and cultured for 21 days. As shown in the upper part of FIG. 2, many transformation foci were observed only when the EML4-ALK fusion protein v1-expressing virus was transfected. The scale bar indicates 100 μm. Further, the same transfected 3T3 cells were inoculated subcutaneously to nude mice at 5×10⁵ cells/mouse and observed for 20 days. It turned out also that tumor was formed only when EML4-ALK fusion protein v1-expressing cells were inoculated. The tumor formation numbers (the number of inoculation sites of 3T3 cells and the number of tumor formation among them) are as follows. The tumor formation number of the full-length ALK expression was 0 among 8, while the tumor formation number in the EML4-ALK fusion protein v1-expressing cells was 8 among 8. In addition, the tumor formation number of EML4-ALK (K589M)-expressing cells was 0 among 8. These results demonstrate that since the full-length ALK protein expression does not induce tumor but the EML4-ALK fusion protein v1 is tumorgenic, the EML4-ALK fusion polynucleotide v1 is a causal gene of cancer. Also, since the tumorgenicity of EML4-ALK was not observed in EML4-ALK (K589M), it would appear that the tumorgenicity was dependent on the kinase activity. Hereinafter, the 3T3 cells modified to express EML4-ALK fusion protein v1 by transfection with the EML4-ALK fusion protein v1 expression plasmid are designated as the v1 expressing 3T3 cells.

Test Example 12 Screening for Inhibitors Against the Kinase Activity of EML4-ALK Fusion Protein

(1) Preparation of EML4-ALK Fusion Protein v1

N-terminally FLAG-tagged EML4-ALK fusion protein v1 was inserted into a vector pMX-iresCD8 capable of co-expression of insert cDNA and cell surface antigen CD8 (J. Biol. Chem., 2001, vol. 276, p. 39012-39020) to create a vector FLAG-EML4-ALKv1/pMX-iresCD8 expressing both FLAG-EML4-ALKv1 and CD8. FLAG-EML4-ALKv1/pMX-iresCD8 was used to create a recombinant retrovirus in the same manner as described above and infected into mouse lymphoid cell line BA/F3 cells. Using a magnetic bead reagent for cell separation and a purification column (anti-CD8 monoclonal antibody immobilized on magnetic beads and a MiniMACS purification column; both are products of Miltenyi Biotec Inc.), cell surface CD8-expressing cells were purified conveniently. The BA/F3 cells expressing this N-terminally FLAG-tagged EML4-ALK fusion protein v1 were cultured in RPMI1640 medium containing 10% fetal bovine serum to obtain 2.7×10⁹ cells. After washing 3 times with PBS, the cells were lysed in a lysis solution (50 mM Tris.HCl (pH 7.4), 150 mM NaCl, 1% Triton X100, 5 mM EDTA, 5 mM EGTA, 1 mM NaVO₄, 1 mM DTT and protease inhibitor cocktail complete). The EML4-ALK fusion protein v1 present in the supernatant obtained after centrifugation was purified using ANTI-FLAG M2 Affinity Gel (SIGMA-ALDRICH Inc.) according to the method described in the product information document.

(2) Detection of the In Vitro Kinase Activity of EML4-ALK Fusion Protein v1

The EML4-ALK fusion protein v1 purified as above was investigated for its phosphorylation activity toward a peptide substrate using a kinase activity detection kit (HTRF KinEASE-TK; Cisbio Inc.). Using TK substrate 1, which was included in the kit, as the substrate, and after adding no ATP or 100 μM ATP, the mixtures were reacted at room temperature for 1 hour, and the count of HTRF was detected as recommended by the Kits manufacturer. As a result, it became clear that the count of HTRF (i.e., phosphorylation of the peptide substrate) was increased by about 12 times by the addition of ATP compare to no addition of ATP. As shown above, the in vitro kinase activity of EML4-ALK fusion protein v1 can be detected using anti-phosphorylated ALK antibody and the kinase activity detection kit.

(3) Inhibitory Effect of Compounds Against the In Vitro Kinase Activity of EML4-ALK Fusion Protein v1

Compounds A, B, C and D, which are known as compounds having an inhibitory effect against ALK, were investigated for their inhibitory effect against the in vitro kinase activity of EML4-ALK fusion protein v1 using the kinase activity detection kit mentioned above. Respective compounds were added to a reaction solution containing the EML4-ALK fusion protein v1 to give a final concentration of 10 μM or 10 nM, followed by reaction with or without the addition of ATP. The rest of the operations were carried out as described in (2) above. In the absence of the compound, the phosphorylation count without ATP addition and with ATP addition was assumed to be 100% inhibition and 0% inhibition, respectively. The inhibition (%) of the kinase activity of EML4-ALK fusion protein v1 by each compound was calculated by the following formula.

[Kinase activity inhibition (%) by a compound]=(1-[phosphorylation count when the compound and ATP were added−phosphorylation count when the compound was not added and ATP was not added]/[phosphorylation count when the compound was not added and ATP was added−phosphorylation count when the compound was not added and ATP was not added])×100

The results obtained are shown in Table 7.

It should be noted that compounds A to D in the table below are those shown in Patent Document 1.

TABLE 7 Final Activity Test compound concentration inhibition (%) Compound A 10 μM 99 Compound B 10 μM 56 Compound C 10 nM 99 Compound D 10 nM 99

It was found that all of the compounds inhibited the phosphorylation activity of the purified EML4-ALK fusion protein v1 on the peptide substrate.

The above results indicated that screening for a substance which inhibits the activity of the protein of the present invention could be performed by preparing the EML4-ALK fusion protein and using the in vitro kinase activity as an index.

Test Example 13 Cell Growth Inhibitory Effect of Inhibitors Against the Kinase Activity of EML4-ALK Fusion Protein on Cells Expressing EML4-ALK Fusion Polynucleotide v1

3×10⁶ cells of v1 expressing 3T3 cells suspended in PBS were inoculated subcutaneously by injection to the back of 5 weeks old male BALB/c nude mice (Charles River Japan, Inc.). After 7 days of the inoculation, the administration of compound C, an inhibitor against the kinase activity of EML4-ALK fusion protein, was initiated. The test was conducted in the solvent group and compound C group, 4 animals per group. Compound C was dissolved in a solvent composed of 10% 1-methyl-2-pyrrolidinone (SIGMA-ALDRICH Inc.)/90% polyethylene glycol 300 (Fluka Inc.) and administered orally at a dose of 10 mg/kg. Administrations were performed once a day for 14 days, and body weight and tumor size were measured every other day. Tumor volume was calculated using the following formula.

[Tumor volume (mm³)]=[Tumor major axis (mm)]×[tumor minor axis (mm)]²×0.5

Assuming that the tumor volume of the solvent group on the day of starting and the day of finishing administration was 100% inhibition and 0% inhibition, respectively, the inhibition rate of compound C was calculated. The results indicated that compound C inhibited the growth of v1 expressing 3T3 cells (tumor) by 103%.

The antitumor effect of compound D was investigated by the similar procedure with the following exceptions. The administration of the compound was started after 6 days of the inoculation and carried out once a day for 10 days. As a result, compound D inhibited the growth of v1 expressing 3T3 cells (tumor) by 101%.

Test Example 14 Inhibitory Effect of Compounds Against the In Vitro Kinase Activity of EML4-ALK Fusion Protein v1

In the same manner as shown in Test Example 12(3), compounds were investigated for their inhibitory effect against the in vitro kinase activity of EML4-ALK fusion protein v1 using the kinase activity detection kit mentioned above. Test compounds were each added to a reaction solution containing the EML4-ALK fusion protein v1 to give 8 final concentrations from 1000 nM to 0.3 nM, followed by addition of ATP. Another reaction solution was prepared to contain the EML4-ALK fusion protein v1 but no test compound (in which the solvent DMSO alone was added at 0.4% in place of the test compound), followed by reaction with or without ATP addition. The rest of the operations were carried out as described in Test Example 12(2). In the absence of the test compound, the phosphorylation count without ATP addition and with ATP addition was assumed to be 100% inhibition and 0% inhibition, respectively. The concentration causing 50% inhibition (IC₅₀) was calculated for each test compound by the logistic regression method.

As a result, the compounds of formula (I) were found to inhibit the kinase activity of EML4-ALK fusion protein v1. In particular, some of the compounds of formula (I) showed IC₅₀ values not greater than 1000 nM or 100 nM in the above test. Among them, the compound of Example 1 showed an IC₅₀ value of 42 nM.

As a result of the above tests, the compounds of formula (I) were confirmed to have an inhibitory effect against the kinase activity of EML4-ALK fusion protein v1. This suggests that the compounds of formula (I) can be used as therapeutic agents, e.g., for EML4-ALK fusion gene-positive cancer in one embodiment or for EML4-ALK fusion gene-positive lung cancer in another embodiment.

A pharmaceutical composition which comprises one or more compounds of formula (I) or pharmaceutically acceptable salts thereof as an active ingredient can be prepared in a conventional manner by using a pharmaceutical excipient, a pharmaceutical carrier or other additives commonly used in the art.

Any mode of administration may be used, either oral administration in the dosage form of tablets, pills, capsules, granules, powders, solutions or the like, or parenteral administration in the dosage form of injections (e.g., intraarticular, intravenous, intramuscular, and the like), suppositories, eye drops, eye ointments, percutaneous solutions, ointments, percutaneous patches, transmucosal solutions, transmucosal patches, inhalants or the like.

Solid compositions used for oral administration include tablets, powders, granules, and the like. In these solid compositions, one or more active ingredients are mixed with at least one inert excipient, for example, lactose, mannitol, glucose, hydroxypropylcellulose, microcrystalline cellulose, starch, polyvinylpyrrolidone, and/or magnesium aluminometasilicate, or the like. The compositions may also comprise inert additives, for example, lubricants (e.g., magnesium stearate and the like), disintegrating agents (e.g., carboxymethyl starch sodium and the like), stabilizers, and/or solubilizers, as in the usual cases. Tablets or pills may be coated with sugar coating or a gastric or enteric film, if necessary.

Liquid compositions for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, elixirs, or the like, and comprise commonly-used inert diluents such as purified water or ethanol. These liquid compositions may comprise, in addition to inert diluents, auxiliaries (e.g., solubilizers, wetting agents, suspending agents, and the like), sweeteners, flavors, aromatics, and/or antiseptics.

Injections for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions or emulsions. Examples of aqueous solvents include injectable distilled water or physiological saline. Examples of non-aqueous solvents include propylene glycol, polyethylene glycol or vegetable oils (e.g., olive oil and the like), as well as alcohols (e.g., ethanol and the like) or Polysorbate 80 (pharmacopoeia name), and the like. These compositions may further comprise isotonizing agents, antiseptics, wetting agents, emulsifiers, dispersants, stabilizers or solubilizers. They are sterilized, for example, by filtration through a bacteria-retaining filter, by incorporation with disinfectants or by irradiation. Alternatively, they may be formulated into a sterile solid composition and reconstituted for use by being dissolved or suspended in sterile water or a sterile injectable solvent before use.

Formulations for external use include ointments, plasters, creams, jellies, cataplasms, sprays, lotions, eye drops, eye ointments, and the like. They comprise commonly-used ointment bases, lotion bases, aqueous or non-aqueous solutions, suspensions, emulsions or the like. Examples of ointment or lotion bases include polyethylene glycol, propylene glycol, white petrolatum, white beeswax, polyoxyethylene hydrogenated castor oil, glycerine monostearate, stearyl alcohol, cetyl alcohol, Lauromacrogol, sorbitan sesquioleate, and the like.

Transmucosal formulations such as inhalants or transnasal formulations are used in solid, liquid or semi-solid form and can be prepared in a conventionally known manner. For example, such formulations may be supplemented as appropriate with known excipients and further with pH adjustors, antiseptics, surfactants, lubricants, stabilizers, thickeners and so on. For their administration, an appropriate device for inhalation or insufflation may be used. For example, using a known device (e.g., a metered-dose inhalation device and the like) or a nebulizer, the compound(s) may be administered alone or as a powder of a formulated mixture or as a solution or suspension in combination with a pharmaceutically acceptable carrier. Dry powder inhalators or the like may be for single or multiple administration use, and dry powders or powder-containing capsules may be used in such devices. Alternatively, they may be in the form of pressurized aerosol sprays or the like which use an appropriate propellant, for example, a preferred gas such as chlorofluoroalkane, hydrofluoroalkane, carbon dioxide, or the like.

In general, for oral administration, the daily dosage is desirably about 0.001 to 100 mg/kg, preferably 0.1 to 30 mg/kg, and more preferably 0.1 to 10 mg/kg body weight, given as a single dose or in 2 to 4 divided doses. For intravenous administration, the daily dosage is desirably about 0.0001 to 10 mg/kg body weight, given in one or several doses per day. Likewise, for transmucosal formulations, the daily dosage is about 0.001 to 100 mg/kg body weight, given in one or several doses per day. The dosage may be determined as appropriate for each case in consideration of symptom, age, sex and so on.

The compounds of formula (I) can be used in combination with various therapeutic or prophylactic agents for diseases against which the compounds of formula (I) would be effective. In general, when an antitumor agent is administered alone during chemotherapy for tumor, particularly malignant tumor, the antitumor agent has a limit in its effect in terms of side effects and the like, and thus often fails to produce a sufficient antitumor effect. For this reason, in clinical cases, multidrug therapy is used in which two or more drugs with different mechanisms of action are combined. By combining antitumor agents with different mechanisms of action, this combination therapy aims to reduce side effects and/or enhance the desired antitumor effect, for example, 1) to reduce the number of non-sensitive cell population, 2) to prevent or delay the development of drug resistance, 3) to disperse toxicity by combination of drugs with different toxicity levels, and the like. In such combination therapy, drugs may be administered simultaneously or separately in succession or at desired time intervals. Formulations for simultaneous administration may be in either mixed or separate form.

Drugs which can be combined include chemotherapeutics (e.g., alkylating agent, antimetabolite, and the like), immunotherapeutic agents, hormonal therapeutic agents, and cell growth factor inhibitors, more specifically drugs such as cisplatin, carboplatin, paclitaxel, docetaxel, gemcitabine, irinotecan, vinorelbine, bevacizumab, and the like.

EXAMPLES

How to prepare the compounds of formula (I) will be further explained in more detail by way of the following examples. It should be noted that the present invention is not limited to the compounds shown in the following examples. In addition, how to prepare the starting compounds is shown in preparation examples. Processes for preparing the compounds of formula (I) are not limited only to those actually shown in the following examples, and the compounds of formula (I) may also be prepared by any combination of these processes or by any process obvious to those skilled in the art.

In the examples, preparation examples and tables shown below, the following abbreviations are used as needed.

Rex: Preparation Example No., Ex: Example No., No: Compound No., Structure: chemical structural formula, Data: physical and chemical data (FAB+: FAB−MS[M+H]⁺, FAB−: FAB−MS[M−H]⁻, ESI+: ESI−MS[M+H]⁺, CI+: CI[M+H]⁺, EI: EI[M]⁺, NMR-DMSO-d6: δ (ppm) of ¹H-NMR peaks in dimethyl sulfoxide-d₆, NMR-CDCl₃: δ (ppm) of ¹H-NMR peaks in chloroform-d, MP: melting point (° C.), Amrph: which means that the intended compound was in amorphous form, Cryst: which means that the intended compound was in crystal form, Salt: salt (if empty, the intended compound is in free form), CL1: monohydrochloride, CL2: dihydrochloride, CL3: trihydrochloride, FM: difumarate, Me: methyl, Et: ethyl, ^(i)Pr: isopropyl, tBu: tert-butyl, nBu: n-butyl. Rsyn and Syn: preparation process (the number indicated means that the intended compound was prepared from corresponding starting materials in the same manner as used for a compound, in which the indicated number represents its Preparation Example No. or Example No.).

Preparation Example 1

To a mixture of propane-2-thiol (1.5 mL), potassium carbonate (3 g) and N,N-dimethylformamide (20 mL), 4-chloro-2-fluoronitrobenzene (2.5 g) was added and stirred at room temperature for 5 hours. After addition of water, the reaction mixture was extracted with ethyl acetate, and the extract was washed with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure to give 4-chloro-2-isopropylsulfanyl-1-nitrobenzene (3.30 g) as a yellow oil.

Preparation Example 2

To a mixture of sodium isopropylsulfinate (3.3 g) and N-methyl-2-pyrrolidinone (20 mL), 2,3-dichloronitrobenzene (4 g) was added and stirred overnight at 70° C. The reaction mixture was diluted with water, and the precipitated solid was collected by filtration and washed with diethyl ether to give 3-chloro-2-isopropylsulfonyl-1-nitrobenzene (3.0 g) as a white solid.

Preparation Example 3

To a mixture of m-chloroperbenzoic acid (7.89 g) and chloroform (100 mL), a mixture of the compound of Preparation Example 1 (3.3 g) and chloroform (50 mL) was added and stirred at 50° C. for 7 hours. After cooling, the reaction mixture was diluted with saturated aqueous sodium hydrogen carbonate and extracted with chloroform. After the organic layer was dried over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=3:1-2:1) to give 4-chloro-2-isopropylsulfonyl-1-nitrobenzene (3.33 g) as a yellow solid.

Preparation Example 4

To a mixture of 2-nitrobenzenesulfonyl chloride (5.09 g), N-methylethylamine (1.35 g) and chloroform (100 mL), triethylamine (4.11 mL) was added under ice cooling and stirred at room temperature for 5 hours. The reaction mixture was diluted with water and extracted with chloroform. The organic layer was washed sequentially with 1 M hydrochloric acid, saturated aqueous sodium hydrogen carbonate and saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to give N-ethyl-N-methyl-2-nitrobenzenesulfonamide (6.48 g) as a brown oil.

Preparation Example 5

To a mixture of N-cyclopropyl-2-nitrobenzenesulfonamide (5.63 g), potassium carbonate (4.82 g) and N,N-dimethylformamide (60 mL), methyl iodide (2.17 mL) was added and stirred at room temperature for 6 hours. The reaction mixture was evaporated under reduced pressure, and the residue was diluted with water and extracted with ethyl acetate. The organic layer was washed with saturated aqueous sodium chloride and dried over anhydrous magnesium sulfate, followed by distilling off the solvent under reduced pressure. The residue was purified by silica gel column chromatography (eluent; n-hexane:diethyl ether=1:0-1:1) to give N-cyclopropyl-N-methyl-2-nitrobenzenesulfonamide (5.35 g) as a brown solid.

Preparation Example 6

To a mixture of the compound of Preparation Example 3 (3.3 g) and acetic acid (30 mL), iron powder (2.23 g) was added and stirred at 80° C. for 3 hours. Insoluble materials in the reaction mixture were removed and the solvent was distilled off under reduced pressure. The residue was diluted with ethyl acetate (100 mL) and insoluble materials were removed, followed by washing with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=3:1-2:1) to give 4-chloro-2-isopropylsulfonylaniline (2.79 g) as a light-orange solid.

Preparation Example 7

To a mixture of 2,4-dichloro-6-methoxy-1,3,5-triazine (370 mg) and tetrahydrofuran (10 mL), a mixture of 2-(isopropylsulfonyl)aniline (400 mg), N-ethyl-N-isopropylpropane-2-amine (0.72 mL) and tetrahydrofuran (5 mL) was added and stirred overnight at room temperature and further stirred at 70° C. for 7 hours. The reaction mixture was cooled on ice and diluted with water (60 mL). The precipitated solid was collected by filtration, purified by silica gel column chromatography (eluent; chloroform) and then washed with hexane to give 4-chloro-N-[2-(isopropylsulfonyl)phenyl]-6-methoxy-1,3,5-triazine-2-amine (200 mg) as a white solid.

Preparation Example 8

To a mixture of 2-(isopropylsulfonyl)aniline (450 mg) and N,N-dimethylformamide (10 mL), 55% sodium hydride in oil (200 mg) was added under ice cooling and stirred for 30 minutes, followed by addition of 2,4-dichloroquinazoline (500 mg). The reaction mixture was stirred for 30 minutes under ice cooling and further stirred overnight at room temperature. The reaction mixture was cooled on ice, diluted with water and then extracted with ethyl acetate. The organic layer was washed with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=5:1-3:1) to give 2-chloro-N-[2-(isopropylsulfonyl)phenyl]quinazoline-4-amine (0.67 g) as a light-yellow solid.

Preparation Example 9

To a mixture of 2-fluoroaniline (232 mg), 2,4-dichloroquinazoline (400 mg) and N,N-dimethylformamide (4 mL), potassium carbonate (430 mg) was added and stirred at room temperature for 8 hours. The reaction mixture was diluted with water (40 mL), and the precipitated solid was collected by filtration and purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=5:1-3:1) to give 2-chloro-N-(2-fluorophenyl)quinazoline-4-amine (0.22 g) as a light-yellow solid.

Preparation Example 10

To a mixture of the compound of Preparation Example 24 (309 mg) and acetonitrile (5 mL), azetidine hydrochloride (112 mg) and N-ethyl-N-isopropylpropane-2-amine (0.42 mL) were added and stirred at room temperature for 1 hour. The reaction mixture was diluted with water, and the precipitated solid was collected by filtration and dried to give 4-azetidin-1-yl-6-chloro-N-(2-fluorophenyl)-1,3,5-triazine-2-amine (253 mg) as a white solid.

Preparation Example 11

To a mixture of tert-butyl 4-hydroxypiperidine-1-carboxylate (1.49 g) and tetrahydrofuran (30 mL), potassium tert-butoxide (830 mg) was added under ice cooling and stirred for 30 minutes, followed by addition of a mixture of 4-fluoro-2-methoxy-1-nitrobenzene (1.00 g) and tetrahydrofuran (20 mL). After stirring at room temperature for 3 hours, the reaction mixture was extracted by addition of water and ethyl acetate, washed with saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:diethyl ether=4:1) to give tert-butyl 4-(3-methoxy-4-nitrophenoxy)piperidine-1-carboxylate (898 mg).

Preparation Example 12

To a mixture of 4-fluoro-2-methoxy-1-nitrobenzene (5 g), potassium carbonate (10 g) and N,N-dimethylformamide (50 mL), 1,4-dioxa-8-azaspiro[4.5]decane (5 g) was added and stirred overnight at 70° C. The reaction mixture was diluted with water (150 mL), and the precipitated solid was collected by filtration and washed with diethyl ether to give 8-(3-methoxy-4-nitrophenyl)-1,4-dioxa-8-azaspiro[4.5]decane (7.86 g) as a light-yellow solid.

Preparation Example 13

To a mixture of tert-butyl 3-(4-methylpiperazin-1-yl)pyrrolidine-1-carboxylate (3.04 g) and chloroform (30 mL), trifluoroacetic acid (10 mL) was added and stirred at room temperature for 1 hour. The reaction mixture was evaporated under reduced pressure to remove the solvent, followed by addition of a mixture of 4-fluoro-2-methoxy-1-nitrobenzene (1.93 g), potassium carbonate (12.2 g) and N,N-dimethylformamide (60 mL). After stirring overnight at 80° C., the reaction mixture was evaporated under reduced pressure to remove the solvent, and the residue was diluted with water and extracted with ethyl acetate. The organic layer was washed with saturated aqueous sodium chloride, and then dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure to give 1-[1-(3-methoxy-4-nitrophenyl)pyrrolidin-3-yl]-4-methylpiperazine (2.16 g).

Preparation Example 14

To a mixture of the compound of Preparation Example 57 (6.68 g), 1-methylpiperazine (4.17 mL) and dichloromethane (100 mL), sodium triacetoxyborohydride (8.04 g) was added and stirred overnight at room temperature. The reaction mixture was diluted with water and saturated aqueous sodium hydrogen carbonate, extracted with chloroform, and then washed with saturated aqueous sodium chloride. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1) to give 1-[1-(3-ethoxy-4-nitrophenyl)piperidin-4-yl]-4-methylpiperazine (6.68 g).

Preparation Example 15

To a mixture of 4-fluoro-2-methyl-1-nitrobenzene (3.08 g), potassium carbonate (6.80 g) and N,N-dimethylformamide (30 mL), piperidine-4,4-diol hydrochloride (3.83 g) was added and stirred at 70° C. for 2 days. The reaction mixture was evaporated under reduced pressure, and the residue was diluted with water and ethyl acetate. The precipitated solid was collected by filtration. After drying, dichloromethane (56 mL), 1-methylpiperazine (3.00 mL) and sodium triacetoxyborohydride (5.75 g) were added and stirred overnight at room temperature. The reaction mixture was diluted with water and saturated aqueous sodium hydrogen carbonate, extracted with chloroform, and then washed with saturated aqueous sodium chloride. After drying over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1) to give 1-methyl-4-[1-(3-methyl-4-nitrophenyl)piperidin-4-yl]piperazine (1.29 g).

Preparation Example 16

To a mixture of concentrated sulfuric acid (40 mL) and acetic acid (60 mL), N-[2-(4-chlorophenyl)ethyl]-2,2,2-trifluoroacetamide (14.2 g) and paraformaldehyde (2.79 g) were added sequentially and stirred overnight under an argon atmosphere. The reaction mixture was added to ice-cold water, extracted with ethyl acetate, and then washed with saturated aqueous sodium hydrogen carbonate and saturated aqueous sodium chloride. After drying over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure to give 7-chloro-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (13.7 g) as a light-yellow solid.

Preparation Example 17

The compound of Preparation Example 16 (13.7 g) was dissolved in concentrated sulfuric acid (60 mL) and then cooled to 0° C., followed by dropwise addition of a solution of potassium nitrate (3.3 g) in concentrated sulfuric acid (60 mL) over 1 hour. After stirring for 1 hour under ice cooling, the reaction mixture was added to ice-cold water. After extraction with ethyl acetate, the organic layer was washed with saturated aqueous sodium chloride and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, and the residue was recrystallized from ethyl acetate-hexane to give 7-chloro-6-nitro-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (4.46 g) as a colorless solid.

Preparation Example 18

To a mixture of tert-butyl 1-oxa-4,9-diazaspiro[5.5]undecane-9-carboxylate (5.80 g) and dioxane (100 mL), 1 M aqueous sodium hydroxide (24.9 mL) and benzyl chloroformate (3.55 mL) were added sequentially under ice cooling and stirred at room temperature for 16 hours. The reaction mixture was concentrated under reduced pressure and then extracted with ethyl acetate. The organic layer was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=9:1-1:1) to give 4-benzyl 9-tert-butyl 1-oxa-4,9-diazaspiro[5.5]undecane-4,9-dicarboxylate (8.06 g) as a colorless syrup.

Preparation Example 19

To a mixture of the compound of Preparation Example 18 (8.06 g) and ethanol (200 mL), 4 M hydrochloric acid in dioxane (30 mL) was added and stirred at room temperature for 16 hours. The solvent was distilled off under reduced pressure, and the residue was recrystallized from diethyl ether-ethanol to give benzyl 1-oxa-4,9-diazaspiro[5.5]undecane-4-carboxylate hydrochloride (3.86 g) as a colorless solid.

Preparation Example 20

To a mixture of the compound of Preparation Example 12 (7.83 g) and ethanol (100 mL), 10% palladium on carbon (water content: 53%, 2.83 g) was added and stirred overnight at room temperature under a hydrogen atmosphere at normal pressure. After filtration through celite, the filtrate was evaporated under reduced pressure to give 4-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)-2-methoxyaniline (6.83 g) as a light-purple solid.

Preparation Example 21

To a mixture of the compound of Preparation Example 71 (1.32 g) and acetic acid (30 mL), iron powder (0.79 g) was added and stirred at 80° C. for 3 hours. Insoluble materials in the reaction mixture were removed and the solvent was distilled off under reduced pressure. The residue was diluted with ethyl acetate and insoluble materials were removed, followed by washing with saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure to give 2-chloro-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]aniline (290 mg).

Tables 8 and 9 show the chemical structures of the compounds prepared in the above preparation examples. Further, in the same manner as shown in the above preparation examples, the additional compounds shown in Tables 10 to 16 were also prepared from their corresponding starting materials. Tables 17 to 19 show the instrumental analysis data of these compounds obtained in the preparation examples.

Example 1

To a mixture of 2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]aniline (230 mg) and ethanol (3 mL), methanesulfonic acid (0.11 mL) was added and stirred at room temperature for 15 minutes, followed by addition of the compound of Preparation Example 8 (200 mg) and further stirring at 100° C. for 3 hours. After cooling, the reaction mixture was diluted with water (20 mL) and adjusted to pH 8 with saturated aqueous sodium hydrogen carbonate, followed by filtration to collect the precipitated solid. The resulting solid was purified by silica gel column chromatography (eluent; chloroform:methanol:saturated aqueous ammonia=50:1:0.1-30:1:0.1) to give N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}quinazoline-2,4-diamine (0.26 g) as a yellow amorphous substance.

Example 2

To a mixture of 2-methoxy-N⁴-methyl-N⁴-(1-methylpiperidin-4-yl)benzene-1,4-diamine (150 mg) and ethanol (3 mL), methanesulfonic acid (0.08 mL) was added and stirred at room temperature for 15 minutes, followed by addition of the compound of Preparation Example 8 (240 mg) and further stirring at 100° C. for 3 hours. After cooling, the reaction mixture was adjusted to pH 8 by addition of water and saturated aqueous sodium hydrogen carbonate, and then extracted with ethyl acetate. The extract was washed with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol:saturated aqueous ammonia=100:1:0.1-50:1:0.1) to give a brown amorphous substance. The resulting amorphous substance was dissolved in ethanol (5 mL) and ethyl acetate (5 mL), followed by addition of 4 M hydrogen chloride in ethyl acetate (0.3 mL). After stirring for 10 minutes, ethyl acetate (20 mL) was added, and the precipitated solid was collected by filtration to give N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[methyl(1-methylpiperazin-4-yl)amino]phenyl}quinazoline-2,4-diamine trihydrochloride (0.15 g) as a light-yellow solid.

Example 3

To a mixture of the compound of Preparation Example 27 (200 mg), 2-methoxy-4-(morpholin-4-yl)aniline (158 mg) and acetonitrile (10 mL), N-ethyl-N-isopropylpropane-2-amine (0.13 mL) was added and heated under reflux for 12 hours. The reaction mixture was diluted with water, and the precipitated solid was collected by filtration, dried and then purified by silica gel column chromatography (eluent; chloroform:methanol=20:1) to give 2-({4-[(2-methoxy-4-morpholin-4-ylphenyl)amino]-1,3,5-triazin-2-yl}amino)-N-methylbenzamide (135 mg) as a white solid.

Example 4

A mixture of the compound of Preparation Example 22 (209 mg), 2-methoxy-4-(4-phenylpiperazin-1-yl)aniline (189 mg), N-ethyl-N-isopropylpropane-2-amine (0.12 mL) and N-methyl-2-pyrrolidinone (3 mL) was stirred at 120° C. for 20 minutes using a microwave reaction system. The reaction mixture was diluted with water, and the precipitated solid was collected by filtration, dried and then purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1). The resulting product was dissolved in ethyl acetate and 4 M hydrogen chloride in ethyl acetate was added thereto, followed by evaporation under reduced pressure to remove the solvent. The residue was crystallized from ethanol to give N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(4-phenylpiperazin-1-yl)phenyl]-1,3,5-triazine-2,4-diamine trihydrochloride (273 mg).

Example 5

To a mixture of the compound of Preparation Example 34 (850 mg) and acetonitrile (17 mL), the compound of Preparation Example 37 (806 mg) and N-ethyl-N-isopropylpropane-2-amine (0.44 mL) were added at room temperature and stirred for 1 hour. The reaction mixture was diluted with water, extracted with chloroform, and then washed with saturated aqueous sodium chloride. After drying over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=1:1). The resulting product was dissolved in ethyl acetate and 4 M hydrogen chloride in ethyl acetate was added thereto, followed by evaporation under reduced pressure to remove the solvent. The residue was crystallized from a mixed solvent of ethanol and ethyl acetate to give 6-chloro-N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(piperidin-4-yloxy)phenyl]-1,3,5-triazine-2,4-diamine hydrochloride (323 mg).

Example 6

A mixture of the compound of Preparation Example 78 (320 mg), the compound of Preparation Example 20 (260 mg), N-ethyl-N-isopropylpropane-2-amine (0.17 mL) and N-methyl-2-pyrrolidinone (1 mL) was reacted at 120° C. for 20 minutes using a microwave reaction system. After cooling, the reaction mixture was poured into water (20 mL), and the precipitated solid was collected by filtration and then dried to give a light-purple solid. To the resulting solid, acetic acid (2 mL) and water (1 mL) were added and stirred overnight at 70° C. The reaction mixture was concentrated under reduced pressure, and the residue was partitioned by addition of ethyl acetate (50 mL) and saturated aqueous sodium hydrogen carbonate (25 mL). The organic layer was washed with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; n-hexane:ethyl acetate=1:1-1:3) to give N-isopropyl-2-[(4-{[2-methoxy-4-(4-oxopiperidin-1-yl)phenyl]amino}-1,3,5-triazin-2-yl)amino]benzenesulfonamide (0.32 g) as an amorphous substance.

Example 7

A mixture of the compound of Example 163 (174 mg), acetic acid (2 mL) and water (1 mL) was stirred overnight at 70° C. To the reaction mixture, ethyl acetate and saturated aqueous sodium hydrogen carbonate were added, and the organic layer was washed with saturated aqueous sodium chloride. After drying over anhydrous magnesium sulfate, the solvent was distilled off under reduced pressure, and the resulting residue was dissolved in dichloromethane (5 mL), followed by addition of 1-methylpiperazine (0.063 mL) and sodium triacetoxyborohydride (122 mg). After stirring at room temperature for two days, the reaction mixture was diluted with saturated aqueous sodium hydrogen carbonate, extracted with chloroform, and then dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1) to give 2-{[4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-N-methylbenzenesulfonamide (42 mg) as a colorless solid.

Example 8

To a mixture of the compound of Example 31 (76 mg) and acetonitrile (5 mL), pyrrolidine (0.041 mL) was added and heated under reflux for 1 hour. The reaction mixture was diluted with water, and the precipitated solid was collected by filtration, dried and then purified by silica gel column chromatography (eluent; chloroform:methanol=20:1) to give 2-({4-[(2-methoxy-4-morpholin-4-ylphenyl)amino]-6-pyrrolidin-1-yl-1,3,5-triazin-2-yl}amino)-N-methylbenzamide (46 mg) as a white powder.

Example 9

To a mixture of the compound of Example 68 (3.15 g) and ethyl acetate (30 mL), 4 M hydrogen chloride in ethyl acetate (30 mL) was added and stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and the residue was diluted with saturated aqueous sodium hydrogen carbonate and then extracted with ethyl acetate. The organic layer was washed with saturated aqueous sodium chloride and then dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol:saturated aqueous ammonia=100:10:1) to give N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(piperidin-4-yloxy)phenyl]-1,3,5-triazine-2,4-diamine (2.1 g) as a colorless amorphous substance.

Example 10

To a mixture of the compound of Example 62 (140 mg), morpholine (0.08 mL) and 1,2-dichloroethane (2 mL), sodium triacetoxyborohydride (80 mg) was added and stirred at room temperature for 5 hours. The reaction mixture was diluted with saturated aqueous sodium hydrogen carbonate and extracted with chloroform, and the organic layer was dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=100:0-100:1) and then washed with diethyl ether to give N⁴-[2-(isopropylsulfonyl)phenyl]-N²-[2-methoxy-4-(4-morpholin-4-ylpiperidin-1-yl)phenyl]quinazoline-2,4-diamine (0.1 g) as a yellow powder.

Example 11

To a mixture of the compound of Example 66 (150 mg), triethylamine (0.05 mL) and tetrahydrofuran (2 mL), acetic anhydride (0.03 mL) was added and stirred at room temperature for 6 hours. The reaction mixture was diluted with saturated aqueous sodium hydrogen carbonate and extracted with ethyl acetate, and the organic layer was washed with water and saturated aqueous sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=100:1-50:1) and then washed with hexane to give N²-[4-(4-acetylpiperazin-1-yl)-2-methoxyphenyl]-N⁴-[2-(isopropylsulfonyl)phenyl]quinazoline-2,4-diamine (0.11 g) as a yellow powder.

Example 12

To a mixture of the compound of Example 147 (240 mg), formalin (0.18 mL) and 1,2-dichloroethane (5 mL), sodium triacetoxyborohydride (280 mg) was added and stirred at room temperature for 3 days. The reaction mixture was diluted with saturated aqueous sodium hydrogen carbonate and extracted with chloroform, and the organic layer was dried over anhydrous sodium sulfate. After the solvent was distilled off under reduced pressure, the residue was purified by silica gel column chromatography (eluent; chloroform:methanol:saturated aqueous ammonia=50:1:0.1-30:10:1) and then washed with diethyl ether to give N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-methyl-diazaspiro[5.5]undecan-9-yl)phenyl]-1,3,5-triazine-2,4-diamine (135 mg) as a light-yellow powder.

Example 13

A mixture of the compound of Example 22 (169 mg) and 6 M hydrochloric acid (4 mL) was stirred at 50° C. for 2 hours. After cooling, the reaction mixture was basified by addition of water and 1 M aqueous sodium hydroxide, and then extracted with chloroform. The organic layer was washed with saturated aqueous sodium chloride and dried over anhydrous magnesium sulfate. The solvent was distilled off under reduced pressure, followed by recrystallization from ethanol-diethyl ether to give 6-{[2-(isopropylsulfonyl)phenyl]amino}-4-({2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}amino)-1,3,5-triazin-2(1H)-one (27 mg).

Example 14

A mixture of the compound of Example 23 (250 mg) and pyridine hydrochloride (1 g) was stirred at 200° C. for 10 minutes. After cooling to room temperature, the reaction mixture was diluted with water and washed with chloroform. The aqueous layer was evaporated under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-10:1) to give 2-[(4-{[2-(isopropylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-5-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenol (45 mg).

Example 15

To a mixture of the compound of Example 102 (630 mg), ethanol (10 mL) and tetrahydrofuran (10 mL), 10% palladium on carbon (water content: 53%, 500 mg) was added and stirred at room temperature for 2 hours under a hydrogen atmosphere at normal pressure. After filtration through celite, the filtrate was evaporated under reduced pressure, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1) to give N-[2-(isopropylsulfonyl)phenyl]-N′-[2-methoxy-4-(1-oxa-4,9-diazaspiro[5.5]undecan-9-yl)phenyl]-1,3,5-triazine-2,4-diamine (102 mg).

Example 16

To a mixture of the compound of Example 177 (1.09 g) and ethyl acetate (10 mL), 4 M hydrogen chloride in ethyl acetate (10 mL) was added and stirred at room temperature for 30 minutes. The reaction mixture was evaporated under reduced pressure, and the residue was washed with ethyl acetate, collected by filtration and then dried to give N-(7-fluoro-1,2,3,4-tetrahydroisoquinolin-6-yl)-N′-[2-(isopropylsulfonyl)phenyl]-1,3,5-triazine-2,4-diamine dihydrochloride (950 mg) as a colorless solid.

Example 17

To a mixture of the compound of Example 178 (1.2 g) and methanol (15 mL), 2 M hydrochloric acid (15 mL) was added and heated overnight under reflux. The reaction mixture was concentrated under reduced pressure, diluted with saturated aqueous sodium hydrogen carbonate, extracted with chloroform and then washed with saturated aqueous sodium chloride. The solvent was distilled off, and the residue was purified by silica gel column chromatography (eluent; chloroform:methanol=1:0-20:1) to give N-(7-chloro-1,2,3,4-tetrahydroisoquinolin-6-yl)-N′-[2-(isopropylsulfonyl)phenyl]-1,3,5-triazine-2,4-diamine (260 mg) as a colorless amorphous substance.

Example 18

To a mixture of the compound of Example 62 (13.6 mg), methylamine hydrochloride (2.0 mg), triethylamine (3.0 mg) and 1,2-dichloroethane (0.5 mL), sodium triacetoxyborohydride (10.5 mg) was added and stirred overnight at room temperature. The reaction mixture was partitioned by addition of chloroform and water, and the organic layer was evaporated under reduced pressure. The residue was separated and purified by HPLC (column: SunFire C18 5 μm 19 mm×100 mm (Waters Inc.), solvent: MeOH/0.1% HCOOH—H₂O=10/90 (0 min)-10/90 (1 min)-95/5 (9 min)-95/5 (12 min), flow rate: 25 mL/min) to give N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(methylamino)piperidin-1-yl]phenyl}quinazoline-2,4-diamine (12.5 mg).

In Examples 76, 77, 78, 85 and 110, the desired compounds were obtained by being reacted in the same manner, and then deprotected, separated and purified.

Example 19

To a mixture of the compound of Example 72 (9.6 mg), cyclohexanone (2.9 mg) and dichloromethane (0.5 mL), sodium triacetoxyborohydride (10.5 mg) was added and stirred overnight at room temperature. The reaction mixture was partitioned by addition of chloroform and water, and the organic layer was evaporated under reduced pressure. The residue was separated and purified by HPLC (column: SunFire C18 5 μm 19 mm×100 mm (Waters Inc.), solvent: MeOH/0.1% HCOOH—H₂O=10/90 (0 min)-10/90 (1 min)-95/5 (9 min)-95/5 (12 min), flow rate: 25 mL/min) to give N-[4-(4-cyclohexylpiperazin-1-yl)-2-methoxyphenyl]-N′-[2-(isopropylsulfonyl)phenyl]-1,3,5-triazine-2,4-diamine (5.1 mg).

In Examples 134 and 141, the desired compounds were obtained by being reacted in the same manner, and then deprotected, separated and purified.

Example 20

To a mixture of the compound of Example 72 (9.6 mg), acetic acid (1.8 mg), 1-hydroxybenzotriazole (3.4 mg) and N,N-dimethylformamide (0.5 mL), PS-Carbodiimide (100 mg, Argonaut Technologies Inc.) was added and stirred overnight at room temperature. After addition of MP-Carbonate (50 mg, Argonaut Technologies Inc) and PS-Isocyanate (50 mg, Argonaut Technologies Inc) at room temperature, the reaction mixture was stirred for 2 hours and filtered to remove insoluble materials. The filtrate was concentrated under reduced pressure, and the residue was separated and purified by HPLC (column: SunFire C18 5 μm 19 mm×100 mm (Waters Inc.), solvent: MeOH/0.1% HCOOH—H₂O=10/90 (0 min)-10/90 (1 min)-95/5 (9 min)-95/5 (12 min), flow rate: 25 mL/min) to give N-[4-(4-acetylpiperazin-1-yl)-2-methoxyphenyl]-N′-[2-(isopropylsulfonyl)phenyl]-1,3,5-triazine-2,4-diamine (6.8 mg).

In Examples 160, 161 and 162, the desired compounds were obtained by being reacted in the same manner, and then deprotected, separated and purified.

Tables 20 to 22 show the chemical structures of the compounds prepared in the above examples. Further, in the same manner as shown in the above examples, the additional compounds shown in Tables 23 to 42 were also prepared from their corresponding starting materials. Tables 43 to 50 show the instrumental analysis data of these compounds obtained in the examples.

TABLE 8 Rex/ Salt Structure  1

 2

 3

 4

 5

 6

 7

 8

 9

10

TABLE 9 Rex/ Salt Structure 11

12

13

14

15

16

17

18

19/ CL1

20

21

TABLE 10 Rex/Salt Structure 22

23

24

25

26

27

28

29

30

31

TABLE 11 Rex/Salt Structure 32

33

34

35

36

37

38

39

40

41

TABLE 12 Rex/Salt Structure 42

43

44

45

46

47

48

49

50

51

TABLE 13 Rex/Salt Structure 52

53

54

55

56

57

58

59

60

61

62

63

TABLE 14 Rex/Salt Structure 64

65

66

67

68

69

70

71

72

73

74

75

TABLE 15 Rex/Salt Structure 76

77

78

79

80

81

82

83

84

85

86

87

TABLE 16 Rex/Salt Structure 88

89

90

91

92

93

94

TABLE 17 Rex Data  1 EI: 230.9  2 CI+: 263.9  3 FAB+: 264.0  4 ESI+: 245.4  5 ESI+: 257.3  6 EI: 232.9  7 ESI+: 343.0  8 ESI+: 362.1  9 ESI+: 274.2 10 FAB+: 280.1 11 FAB+: 353.2 12 ESI+: 295.1 13 FAB+: 321.1 14 FAB+: 349.2 15 ESI+: 319.15 16 EI: 262.9, 265.0 17 EI: 308.0 18 ESI+: 391.1 19 ESI+: 291.1 20 EI: 264.0 21 ESI+: 309.2

TABLE 18 Rex Rsyn Data 22 7 ESI+: 313.1 23 8 ESI+: 396.1 24 7 EI: 259.8 25 7 ESI+: 298.0, 300.0 26 7 ESI+: 225.09 27 7 EI: 264.11 28 8 ESI+: 396.1 29 8 FAB+: 396.0 30 8 EI: 395.0 31 1 EI: 230.9 32 3 CI+: 264.0 33 8 CI+: 396.1 34 7 FAB+: 346.9 35 6 EI: 232.9 36 8 FAB+: 396.0 37 20 EI: 322.1 38 10 ESI+: 398.2 39 1 EI: 226.9 40 3 EI: 258.9 41 1 EI: 226.9 42 6 EI: 228.9 43 3 EI: 258.9 44 8 ESI+: 392.2 45 6 EI: 228.9 46 8 ESI+: 392.2 47 11 FAB+: 254.1 48 20 EI: 223.1 49 6 EI: 232.9 50 7 FAB+: 327.0 51 8 ESI+: 396.1 52 8 ESI+: 313.1 53 8 ESI+: 334.2 54 8 ESI+: 334.1 55 12 ESI+: 314.2 56 20 ESI+: 284.2 57 12 EI: 264.0 58 12 EI: 278.0 59 14 EI+: 363.2 60 20 ESI+: 319.2 61 20 ESI+: 333.2 62 12 ESI+: 392.1 63 12 ESI+: 442.2 64 20 ESI+: 362.0 65 21 ESI+: 412.2 66 7 FAB+: 285.0 67 7 ESI+: 314.1 68 20 ESI+: 289.2

TABLE 19 Rex Rsyn Data 69 20 ESI+: 291.3 70 7 ESI+: 299.2 71 12 ESI+: 338.9, 340.9 72 2 EI+: 227.9 73 6 EI+: 196.9 74 7 ESI+: 314.1 75 7 EI+: 309.9 76 12 EI+: 305.1 77 20 EI+: 275.1 78 7 ESI+: 328.2 79 7 EI+: 341.0 80 7 ESI+: 300.06, 302.04 81 7 ESI+: 342.3 82 6 EI+: 214.0 83 7 ESI+: 340.2, 342.0 84 7 ESI+: 326.0, 327.9 85 7 ESI+: 327.9, 330.0 86 7 ESI+: 356.0, 357.9 87 6 EI+: 226.0 88 7 ESI+: 340.2 89 7 ESI+: 354.3 90 12 ESI+: 225.2 91 20 ESI+: 195.0 92 20 EI+: 278.0 93 7 ESI+: 312.9 94 7 ESI+: 314

TABLE 20 Ex/Salt Structure 1

2/CL3

3

4/CL3

5/CL1

6

7

8

TABLE 21 Ex/Salt Structure  9

10

11

12

13

14

15

16/CL2

TABLE 22 Ex/Salt Structure 17

18

19

20

TABLE 23 Ex/Salt Structure 21

22

23

24

25

26/FM

27

28

TABLE 24 Ex/Salt Structure 29

30

31

32

33

34/CL2

35

36

TABLE 25 Ex/Salt Structure 37

38

39

40

41

42

43

44

TABLE 26 Ex/Salt Structure 45

46

47

48

49

50

51

52/CL3

TABLE 27 Ex/Salt Structure 53/CL1

54

55

56

57

58

59

60

TABLE 28 Ex/ Salt Structure 61

62

63/ CL3

64

65

66

67

68

TABLE 29 Ex/ Salt Structure 69

70/ CL2

71

72

73

74

75

76

TABLE 30 Ex/ Salt Structure 77

78

79

80

81

82

83

84

TABLE 31 Ex/ Salt Structure 85

86

87

88

89

90

91

92

TABLE 32 Ex/ Salt Structure 93

94

95

96

97

98

99

100

TABLE 33 Ex/ Salt Structure 101

102

103

104

105

106

107

108

TABLE 34 Ex/ Salt Structure 109

110

111

112

113

114

115

116

TABLE 35 Ex/ Salt Structure 117

118

119

120

121

122

123

124

TABLE 36 Ex/ Salt Structure 125

126

127

128

129

130

131

132

TABLE 37 Ex/ Salt Structure 133

134

135

136

137

138

139

140

TABLE 38 Ex/ Salt Structure 141

142

143

144

145

146

147

148

TABLE 39 Ex/ Salt Structure 149

150

151

152

153

154

155

156

TABLE 40 Ex/ Salt Structure 157

158

159

160

161

162

163

164

TABLE 41 Ex/ Salt Structure 165

166

167

168

169

170

171

172

TABLE 42 Ex/ Salt Structure 173

174

175

176

177

178

179

180

181

TABLE 43 Ex Data 1 ESI+: 630.3 NMR-CDC13: 1.30 (6H, d, J = 6.9 Hz), 1.65-1.8 (2H, m), 1.96 (2H, d, J = 11.8 Hz), 2.31 (3H, s), 2.35-2.8 (11H, m), 3.2-3.3 (1H, m), 3.68 (2H, d, J = 12.3 Hz), 3.90 (3H, s), 6.58 (2H, m), 7.2-7.3 (2H, m), 7.33 (1H, s), 7.6-7.7 (3H, m), 7.88 (1H, d, J = 8.2 Hz), 7.91 (1H, d, J = 8.0 Hz), 8.45 (1H, d, J = 8.2 Hz), 8.90 (1H, d, J = 8.5 Hz), 10.18 (1H, s) Amrph 2 ESI+: 575.3 3 ESI+: 436.1 4 ESI+: 560.4 5 FAB+: 533.1 6 FAB−: 510.3 7 ESI+: 568.1 NMR-DMSOd6: 1.40-1.59 (2H, m), 1.75-1.91 (2H, m), 2.08-2.19 (3H, m), 2.20-2.60 (12H, m), 2.60-2.78 (2H, m), 3.64-3.85 (5H, m), 6.40-6.55 (1H, m), 6.55-6.69 (1H, br), 7.10-7.30 (2H, m), 7.30-7.90 (2H, m), 8.19-8.42 (2H, m), 8.72-8.98 (1H, m), 8.98-9.16 (1H, m) Amrph 8 ESI+: 505.3 9 FAB+: 499.1 10 ESI+: 617.4 11 ESI+: 575.3 12 ESI+: 566.4 NMR-CDC13: 1.31 (6H, d, J = 6.8 Hz), 1.5-1.7 (8H, m), 2.0-2.15 (2H, m), 2.37 (3H, s), 2.68 (2H, br, 2.99 (2H, t, J = 10.3 Hz), 3.2-3.3 (1H, m), 3.4-3.5 (2H, m), 3.88 (3H, s), 6.55 (2H, br), 7.22 (1H, t, J = 7.8 Hz), 7.63 (2H, br), 7.88 (1H, dd, J = 1.5, 7.8 Hz), 8.10 (1H, br), 8.36 (1H, br), 8.54 (1H, br), 9.28 (1H, s) Cryst (MP: 132-135) 13 ESI+: 597.3 14 FAB+: 567.3 15 ESI+: 554.4 16 ESI+: 443.2 17 ESI+: 459.2, 461.1 18 ESI+: 561 19 ESI+: 566 20 ESI+: 526

TABLE 44 Ex Syn Data 21 1 FAB+: 547.2 22 1 ES+: 611.2 23 1 ES+: 581.2 NMR-CDC13: 1.31 (6H, d, J = 6.8 Hz), 1.65-1.8 (2H, m), 1.97 (2H, d, J = 11.7 Hz), 2.34 (3H, s), 2.3-2.8 (11H, m), 3.2-3.3 (1H, m), 3.70 (2H, d, J = 12.2 Hz), 3.88 (3H, s), 6.54 (2H, m), 7.2 (1H, m), 7 62 (2H, br), 7.88 (1H, dd, J = 1.5, 7.8 Hz), 8.10 (1H, br), 8.37 (1H, br), 8.53 (1H, br), 9.29 (1H, s) Cryst (MP: 164-165) 24 1 ES+: 498.2 NMR-CDC13: 1.31 (6H, d, J = 6.8 Hz), 2.42 (3H, s), 2.68 (4H, br), 3.15-3.35 (5H, m), 3.89 (3H, s), 6.54 (2H, br), 7.2 (1H, m), 7.63 (2H, br), 7.89 (1H, d, J = 6.4 Hz), 8.12 (1H, br), 8.38 (1H, br), 8.53 (1H, br), 9.30 (1H, s) Amrph 25 1 FAB+: 664.2 26 2 FAB+: 454 27 1 ES+: 465 28 1 ES+: 410.3 29 3 ES+: 397.2 30 3 ES+: 431.2 31 3 ES+: 470.1 32 1 ES+: 664.3 33 8 FAB+: 465.2 34 8 FAB+: 468.2 35 1 FAB+: 664.2 36 1 ES+: 664.3 37 1 FAB+: 542.3 38 1 ES+: 664.3 39 3 ES+: 519.2, 521.2 40 8 ES+: 570.4 41 8 ES+: 554.4 42 8 ES+: 556.3 43 1 ES+: 666.4 44 1 ES+: 664.3 45 1 ES+: 630.4 NMR-CDC13: 1.30 (6H, d, J = 6.8 Hz), 1.6-1.7 (2H, m), 1.86 (2H, d, J = 12.7 Hz), 1.97 (2H, t, J = 11.7 Hz), 2.28 (3H, s), 2.3- 2.4 (1H, m), 2.77 (4H, t, J = 4.8 Hz), 2.94 (2H, d, J = 11.7 Hz), 3.19 (4H, t, J = 4.8 Hz), 3.2-3.3 (1H, m), 3.90 (3H, s), 6.57 (1H, d, J = 2.4 Hz), 6.58 (1H, d, J = 2.4 Hz), 7.2-7.4 (3H, m), 7.6-7.7 (3H, m), 7.85-7.95 (2H, m), 8.45 (1H, br), 8.90 (1H, d, J = 8.3 Hz), 10.19 (1H, s) Cryst (MP: 109-114)

TABLE 45 Ex Syn Data 46 1 ESI+: 534.3 47 1 ESI+: 660.4 48 1 ESI+: 660.4 49 1 ESI+: 582.3 50 1 ESI+: 548.3 51 6 ESI+: 497.3 52 2 ESI+: 526.3 NMR-DMSOd6: 1.03-1.21 (6H, m), 1.80-2.05 (2H, m), 2.09- 2.22 (2H, m), 2.60-2.78 (3H, m), 2.85-3.15 (5H, m), 3.35-3.55 (3H, m), 3.83 (3H, s), 3.90-4.08 (1H, m), 4.10-4.70 (3H, br); 6.60-7.28 (1H, br), 7.30-7.90 (4H, m), 8.00-8.50 (2H, m), 9.28- 9.52 (1H, br) 9.52-9.73 (1H, br), 10.51-10.85 (1H, br) Amrph 53 2 ESI+: 500.2 54 1 ESI+: 664.3 55 1 ESI+: 581.4 56 3 ESI+: 590.3 57 3 ESI+ 540.3 58 10  ESI+: 552.3 NMR-DMSOd6: 1:01-1.29 (6H, br), 1.40-1.60 (2H, m), 1.60- 1.78 (4H, m), 1.83-2.00 (2H, m), 2.06-2.20 (1H, m), 2.41-2.62 (4H, m), 2.69-2.82 (2H, m), 3.19-3.52 (1H, m), 3.58-3.71 (2H, m), 3.76 (3H, s), 5.90-6.56 (1H, m), 6.56-6.71 (1H, br), 7.15- 7.35 (2H, m), 7.45-7.62 (1H, m) 7.62-7.89 (1H, m), 8.20-8.45 2H, m), 8.60-9.03 (1H, m), 9.15-9.35 (1H, br) Amrph 59 1 ESI+: 5.95.3 60 10  ESI+: 568.3 61 3 ESI+: 499.3 62 6 FAB+: 546.2 63 ESI+: 568.3 NMR-DMSOd6: 1.05-1.25 (6H, m), 1.78-2.10 (4H, m), 2.10- 2.35 (2H, m), 2.80-4.21 (17H, m), 4.35-4.51 (1H, m), 6.60-7.21 (1H, m), 7.30-7.55 (2H, m), 7.55-7.90 (2H, m), 8.10-8.50 (2H, m), 9.20-9.40 (1H, m), 9.40-9.65 (1H, m), 10.42-11.42 (1H, m) Amrph

TABLE 46 Ex Syn Data 64  1 ESI+: 602.3 65  1 FAB +: 602.3 66  1 ESI+: 533.3 67  1 ESI+: 534.3 68  1 FAB+: 599.2 69 12 ESI+: 513.3 70  2 ESI+: 566.4 71  1 FAB+: 583.3 72  9 ESI+: 484.3 NHR-DMSOd6: 1.02-1.29 (6H, br), 2.73-2.98 (4H, m), 2.98- 3.16 (4H, m), 3.17-3.50 (2H, m), 3.76 (3H, s), 6.40-6.55 (1H, m), 6.44-6.71 (1H, br), 7.18-7.39 (2H, m) 7.40-7.62 (1H, m), 7.62-7.90 (1H, m), 8.20-8.46 (2H, m), 8.58-9.53 (2H, m) Amrph 73 10 ESI+: 644.3 74 18 ESI+: 605 75 18 ESI+: 619 76 18 ESI+: 590 77 18 ESI+: 604 78 18 ESI+: 619 79 18 ESI+: 618 80 18 ESI+: 632 81 18 ESI+: 587 82 18 ESI+: 601 83 18 ESI+: 615 84 18 ESI+: 670 85 18 ESI+: 616 86 18 ESI+: 658 87 18 ESI+: 644 88 18 ESI+: 660 89 18 ESI+: 692 90 18 ESI+: 658 91 18 ESI+: 630 92 18 ESI+: 629 93 18 ESI+: 631 94 18 ESI+: 659 95 18 ESI+: 658 96 18 ESI+: 672 97 18 ESI+: 691 98  1 FAB+: 581.3 99  1 FAB+: 595.3 100  1 FAB+: 609.3 101  3 ESI+: 638.2 102  3 ESI+: 688.4 103  9 ESI+: 538.4 104 18 ESI+: 512 105 18 ESI+: 526 106 18 ESI+: 540 107 18 ESI+: 556

TABLE 47 Ex Syn Data 108 18 ESI+: 570 109 18 ESI+: 572 110 18 ESI+: 541 111 18 ESI+: 544 112 18 ESI+: 538 113 18 ESI+: 595 114 18 ESI+: 673 115 19 ESI+: 609 116 19 ESI+: 611 117 19 ESI+: 645 118 19 ESI+: 649 119  1 ESI+: 553.3 120 12 ESI+: 552.4 NMR-DMSOd6: 1.03-1.22 (6H, m), 1.22-1.41 (2H, m), 1.59- 1.87 (6H, m), 2.10-2.35 (2H, m), 2.60-2.85 (4H, m), 3.22-3.53 (4H, m), 3.76 (3H, s), 6.45-6.58 (1H, m), 6.58-6.70 (1H, m), 7.18-7.37 (2H, m), 7.42-7.61 (1H, m), 7.68-7.86 (1H, m), 8.21-8.42 (2H, m), 8.82-9.05 (1H, m), 9.20-9.31 (1H, m) Amrph 121 12 ESI+: 568.4 122  1 FAB+: 512.3 123 10 FAB+: 582.1 NMR-CDC13: 1.03 (3H, t, J = 7.2 Hz), 1.6-1.7 (2H, m), 1.96 (2H, d, J = 12.2 Hz), 2.30 (3H, s), 2.3-2.8 (13H, m), 2.98 (2H, q, J = 7.2 Hz), 3.87 (3H, s), 4.86 (1H, br), 6.51 (1H, br), 6.53 (1H, d, J = 2.0 Hz), 7.22 (1H, d, J = 7.6 Hz), 7.5-7.7 (2H, m), 7.94 (1H, dd, J = 1.5, 7.8 Hz), 8.06 (1H, d, J = 8.3 Hz), 8.32 (1H, br), 8.40 (1H, br), 8.76 (1H, s) Cryst (MP: 155.160) 124  1 FAB+: 551.2 125  1 FAB+: 565.2 126  1 ESI+: 567.3 127  1 ESI+: 567.4

TABLE 48 Ex Syn Data 128  1 ESI+: 582.3 NMR-CDC13: 1.6-1.8 (2H, m), 1.96 (2H, d, J = 12.2 Hz), 2.30 (3H, s), 2.3-2.8 (10H, m), 2.75 (6H, s), 3.70 (2H, d, J = 12.7 Hz), 3.88 (3H, s), 4.86 (1H, br), 6.5-6.6 (2H, m), 7.15- 7.25 (1H, m), 7.5-7.7 (2H, m), 7.83 (1H, d, J = 7.8 Hz), 8,.12 (1H, br), 8.35 (1H, br), 8.50 (1H, br), 9.11 (1H, s) Cryst (MP: 167-170) 129  1 ESI+: 579.3 130  1 ESI+: 585.4, 587.2 131 19 ESI+: 540 132 19 ESI+: 542 133 19 ESI+: 570 134 19 ESI+: 527 135 19 ESI+: 597 136 19 ESI+: 526 137 19 ESI+: 554 138 19 ESI+: 584 139 19 ESI+: 569 140 19 ESI+: 568 141 19 ESI+: 567 142 19 ESI+: 581 143 19 ESI+: 595 144 19 ESI+: 609 145 19 ESI+: 607 146 19 ESI+: 657 147  1 ESI+: 552.4 148  6 FAB−: 496.1 149 10 ESI+: 596.3 NMR-CDC13: 1.00 (6H, d, J = 6.3 Hz), 1.6-1.8 (2H, m), 1.96 (2H, d, J = 11.7 Hz), 2.31 (3H, s), 2.3-2.8 (11H, m), 3.4-3.5 (1H, m), 3.69 (2H, d, J = 12.2 Ha), 3.88 (3H, s), 4.56 (1H, br), 6.48 (1H, br), 6.54 (1H, d, J = 2.4 Hz), 7.2-7.25 (1H, m), 7.5- 7.7 (2H, m), 7.97 (1H, d, J = 6.8 Hz), 8.08 (1H, d, J = 8.8 Hz), 8.35 (2H, br), 8.61 (1H, s) Cryst (MP: 185-189) 150 20 ESI+: 568 151 20 ESI+: 554 152 20 ESI+: 570 153 20 ESI+: 556 154 20 ESI+: 584 155 20 ESI+: 569 156 20 ESI+: 583 157 20 ESI+: 597

TABLE 49 Ex Syn Data 158 20 ESI+: 611 129 20 ESI+: 583 160 20 ESI+: 584 161 20 ESI+: 555 162 20 ESI+: 569 163  3 ESI+: 528.3 164  3 ESI+: 570.3 165  6 ESI+: 526.4 166 10 ESI+: 610.3 NMR-CDC13: 0.99 (6H, d, J = 6.3 Hz), 1.6-1.8 (2H, m), 1.96 (2H, d, J = 11.7 Hz), 2.31 (3H, s), 2.3-2.8 (11H, m), 2.7 (3H, m), 3.69 (2H, d, J = 12.2 Hz), 3.88 (3H, s), 4.15-4.2 (1H, m), 6.48 (1H, br), 6.54 (2H, br), 7.15-7.2 (1H, m), 7.5-7.6 (1H, m), 7.91 (1H, d, J = 7.8 Hz), 8.11 (1H, d, J = 8.8 Hz), 8.36 (1H, br), 8.42 (1H, br), 8.90 (1H, s) Cryst (MP: 162-164) 167  6 ESI+: 526.6 168 10 ESI+: 610.4 169  1 ESI+: 624.3 170  1 ESI+: 608.3 171  1 ESI+: 594.3 NMR-DMSOd6: 0.28-0.51 (4H, m), 1.41-1.59 (2H, m), 1.76- 1.91 (2H, m), 2.01-2.20 (4H, m), 2.20-2.39 (5H, m), 2.40- 2.60 (4H, m), 2.60-2.76 (2H, m), 3.62-3.85 (5H, m), 6.42-6.55 (1H, m), 6.55-6.70 (1H, m), 7.11-7.50 (2H, m), 7.72-7.89 (1H, m), 8.19-8.43 (1H, m), 8.75-8.95 (1H, m), 9.05-9.20 (1H, m) Amrph 172  1 ESI+: 596.3 173  1 ESI+: 608.3 174  1 ESI+: 622.4 175  1 ESI+: 471.0

TABLE 50 Ex Syn Data 176 10  ESI+: 553.3 NMR-DMSOd6: 1.04-1.29 (6H, br), 2.05-2.58 (11H, m), 3.18-3.50 (2H, m), 3.50-3.66 (2H, m), 3.73 (3H, s), 3.85-4.00 (2H, m), 5.90-6.22 (2H, m), 7.10-7.40 (2H, m), 7.40-7.88 (2H, m), 8.17-8.42 (2H, m), 8.60-9.01 (1H, m), 9.47-9.35 (1H, br) Amrph 177 3 ESI+: 543.2 178 1 ESI+: 555.2, 557.2 179 1 ESI+: 529.2 180 1 ESI+: 581.3 181 1 ESI+: 582

Tables 51 to 95 show the structures of other compounds falling within the present invention. These compounds were synthesized or can be synthesized as described in the above preparation examples or examples, or by any process obvious to those skilled in the art with or without modifications.

It should be noted that the symbols in the tables are as defined below:

—R^(1a′), —R^(1b′), —R^(1c′), —R^(1d′), —R^(2′), —R^(3′), —R^(4′), —R^(5′), —R^(6a′), —R^(6b′), —R^(6c′), —R^(6d′) and —R^(A) correspond to the substituents in the general formulae.

TABLE 51

No —R^(3′) A1 

A2 

A3 

A4 

A5 

A6 

A7 

A8 

A9 

A10

A11

A12

A13

A14

A15

A16

A17

A18

A19

A20

A21

A22

A23

A24

TABLE 52

No —R^(3′) A25

A26

A27

A28

A29

A30

A31

A32

A33

A34

A35

A36

A37

A38

A39

A40

A41

A42

A43

A44

A45

A46

A47

A48

TABLE 53

No —R^(3′) A49

A50

A51

A52

A53

A54

A55

A56

A57

A58

A59

A60

A61

A62

A63

A64

A65

A66

A67

A68

A69

A70

A71

A72

TABLE 54

No —R^(4′) B1 

B2 

B3 

B4 

B5 

B6 

B7 

B8 

B9 

B10

B11

B12

B13

B14

B15

B16

B17

B18

B19

B20

B21

B22

B23

B24

TABLE 55

No —R^(4′) B25

B26

B27

B28

B29

B30

B31

B32

B33

B34

B35

B36

B37

B38

B39

B40

B41

B42

B43

B44

B45

B46

B47

B48

TABLE 56

No —R^(4′) B49

B50

B51

B52

B53

B54

B55

B56

B57

B58

B59

B60

B61

B62

B63

B64

B65

B66

B67

B68

B69

B70

B71

B72

TABLE 57

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) C1  —H —H —H —H —F —H —S(═O)₂iPr C2  —H —H —H —H —Cl —H —S(═O)₂iPr C3  —H —H —H —H —Br —H —S(═O)₂iPr C4  —H —H —H —H —OEt —H —S(═O)₂iPr C5  —H —H —H —H —OiPr —H —S(═O)₂iPr C6  —H —H —H —H —CF₃ —H —S(═O)₂iPr C7  —H —H —H —H —CN —H —S(═O)₂iPr C8  —H —H —H —H Me —H —S(═O)₂iPr C9  —H —H —H —H Et —H —S(═O)₂iPr C10 —H —H —H —H —SMe —H —S(═O)₂iPr C11 —H —H —H —H —OCF₃ —H —S(═O)₂iPr C12 —H —H —H —H —OMe —Cl —S(═O)₂iPr C13 —H —H —H —H —OMe —Br —S(═O)₂iPr C14 —H —H —H —H —OMe Me —S(═O)₂iPr C15 —H —H —H —H —OMe —SMe —S(═O)₂iPr C16 —H —H —H —H —OMe —NMe₂ —S(═O)₂iPr C17 —H —H —H —H —OMe —NEt₂ —S(═O)₂iPr C18 —H —H —H —H —OMe

—S(═O)₂iPr C19 —H —H —H —H —OMe

—S(═O)₂iPr C20 —H —H —H —H —OMe

—S(═O)₂iPr C21 —H —H —H —H —OMe

—S(═O)₂iPr C22 —H —H —H —H —OMe —CN —S(═O)₂iPr C23 —F —H —H —H —OMe —H —S(═O)₂iPr C24 —H —F —H —H —OMe —H —S(═O)₂iPr C25 —H —H —F —H —OMe —H —S(═O)₂iPr C26 —H —H —H —F —OMe —H —S(═O)₂iPr

TABLE 58

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) C27 —Cl —H —H —H —OMe —H —S(═O)₂iPr C28 —H —Cl —H —H —OMe —H —S(═O)₂iPr C29 —H —H —Cl —H —OMe —H —S(═O)₂iPr C30 —H —H —H —Cl —OMe —H —S(═O)₂iPr C31 —Br —H —H —H —OMe —H —S(═O)₂iPr C32 —H —Br —H —H —OMe —H —S(═O)₂iPr C33 —H —H —Br —H —OMe —H —S(═O)₂iPr C34 —H —H —H —Br —OMe —H —S(═O)₂iPr C35 Me —H —H —H —OMe —H —S(═O)₂iPr C36 —H Me —H —H —OMe —H —S(═O)₂iPr C37 —H —H Me —H —OMe —H —S(═O)₂iPr C39 —H —H —H Me —OMe —H —S(═O)₂iPr C40 —OMe —H —H —H —OMe —H —S(═O)₂iPr C41 —H —OMe —H —H —OMe —H —S(═O)₂iPr C42 —H —H —OMe —H —OMe —H —S(═O)₂iPr C43 —H —H —H —OMe —OMe —H —S(═O)₂iPr C44 —CN —H —H —H —OMe —H —S(═O)₂iPr C45 —H —CN —H —H —OMe —H —S(═O)₂iPr C46 —H —H —CN —H —OMe —H —S(═O)₂iPr C47 —H —H —H —CN —OMe —H —S(═O)₂iPr C48 —CF₃ —H —H —H —OMe —H —S(═O)₂iPr C49 —H —CF₃ —H —H —OMe —H —S(═O)₂iPr C50 —H —H —CF₃ —H —OMe —H —S(═O)₂iPr C51 —H —H —H —CF₃ —OMe —H —S(═O)₂iPr C52 —SMe —H —H —H —OMe —H —S(═O)₂iPr C53 —H —SMe —H —H —OMe —H —S(═O)₂iPr C54 —H —H —SMe —H —OMe —H —S(═O)₂iPr C55 —H —H —H —SMe —OMe —H —S(═O)₂iPr C56 —OCF₃ —H —H —H —OMe —H —S(═O)₂iPr C57 —H —OCF₃ —H —H —OMe —H —S(═O)₂iPr C58 —H —H —OCF₃ —H —OMe —H —S(═O)₂iPr C59 —H —H —H —OCF₃ —OMe —H —S(═O)₂iPr

TABLE 59

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) C60 —H —H —H —H —OMe —H —S(═O)₂Et C61 —H —H —H —H —OMe —H —S(═O)₂Me C62 —H —H —H —H —OMe —H —S(═O)₂NHMe C63 —H —H —H —H —OMe —H —S(═O)₂NMe₂ C64 —H —H —H —H —OMe —H —C(═O)NHMe C65 —H —H —H —H —OMe —H —C(═O)NMe₂ C66 —H —H —H —H —OMe —H —C(═O)iPr C67 —H —H —H —H —OMe —H —C(═O)Et C68 —H —H —H —H —OMe —H —F C69 —H —H —H —H —OMe —H —Cl C70 —H —H —H —H —OMe —H —Br C71 —H —H —H —H —OMe —H —OMe C72 —H —H —H —H —OMe —H —OEt C73 —H —H —H —H —OMe —H —OiPr C74 —H —H —H —H —OMe —H —OCF₃ C75 —H —H —H —H —OMe —H —SMe C76 —H —H —H —H —OMe —H —SEt C77 —H —H —H —H —OMe —H —SiPr C78 —H —H —H —H —OMe —H Me C79 —H —H —H —H —OMe —H Et C80 —H —H —H —H —OMe —H iPr C81 —H —H —H —H —OMe —H —CF₃

TABLE 60

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) D1  —H —H —H —H —F —H —S(═O)₂iPr D2  —H —H —H —H —Cl —H —S(═O)₂iPr D3  —H —H —H —H —Br —H —S(═O)₂iPr D4  —H —H —H —H —OEt —H —S(═O)₂iPr D5  —H —H —H —H —OiPr —H —S(═O)₂iPr D6  —H —H —H —H —CF₃ —H —S(═O)₂iPr D7  —H —H —H —H —CN —H —S(═O)₂iPr D8  —H —H —H —H Me —H —S(═O)₂iPr D9  —H —H —H —H Et —H —S(═O)₂iPr D10 —H —H —H —H —SMe —H —S(═O)₂iPr D11 —H —H —H —H —OCF₃ —H —S(═O)₂iPr D12 —H —H —H —H —OMe —Cl —S(═O)₂iPr D13 —H —H —H —H —OMe —Br —S(═O)₂iPr D14 —H —H —H —H —OMe Me —S(═O)₂iPr D15 —H —H —H —H —OMe —SMe —S(═O)₂iPr D16 —H —H —H —H —OMe —NMe₂ —S(═O)₂iPr D17 —H —H —H —H —OMe —NEt₂ —S(═O)₂iPr D18 —H —H —H —H —OMe

—S(═O)₂iPr D19 —H —H —H —H —OMe

—S(═O)₂iPr D20 —H —H —H —H —OMe

—S(═O)₂iPr D21 —H —H —H —H —OMe

—S(═O)₂iPr D22 —H —H —H —H —OMe —CN —S(═O)₂iPr D23 —F —H —H —H —OMe —H —S(═O)₂iPr D24 —H —F —H —H —OMe —H —S(═O)₂iPr D25 —H —H —F —H —OMe —H —S(═O)₂iPr D26 —H —H —H —F —OMe —H —S(═O)₂iPr

TABLE 61

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) D27 —Cl —H —H —H —OMe —H —S(═O)₂iPr D28 —H —Cl —H —H —OMe —H —S(═O)₂iPr D29 —H —H —Cl —H —OMe —H —S(═O)₂iPr D30 —H —H —H —Cl —OMe —H —S(═O)₂iPr D31 —Br —H —H —H —OMe —H —S(═O)₂iPr D32 —H —Br —H —H —OMe —H —S(═O)₂iPr D33 —H —H —Br —H —OMe —H —S(═O)₂iPr D34 —H —H —H —Br —OMe —H —S(═O)₂iPr D35 Me —H —H —H —OMe —H —S(═O)₂iPr D36 —H Me —H —H —OMe —H —S(═O)₂iPr D37 —H —H Me —H —OMe —H —S(═O)₂iPr D39 —H —H —H Me —OMe —H —S(═O)₂iPr D40 —OMe —H —H —H —OMe —H —S(═O)₂iPr D41 —H —OMe —H —H —OMe —H —S(═O)₂iPr D42 —H —H —OMe —H —OMe —H —S(═O)₂iPr D43 —H —H —H —OMe —OMe —H —S(═O)₂iPr D44 —CN —H —H —H —OMe —H —S(═O)₂iPr D45 —H —CN —H —H —OMe —H —S(═O)₂iPr D46 —H —H —CN —H —OMe —H —S(═O)₂iPr D47 —H —H —H —CN —OMe —H —S(═O)₂iPr D48 —CF₃ —H —H —H —OMe —H —S(═O)₂iPr D49 —H —CF₃ —H —H —OMe —H —S(═O)₂iPr D50 —H —H —CF₃ —H —OMe —H —S(═O)₂iPr D51 —H —H —H —CF₃ —OMe —H —S(═O)₂iPr D52 —SMe —H —H —H —OMe —H —S(═O)₂iPr D53 —H —SMe —H —H —OMe —H —S(═O)₂iPr D54 —H —H —SMe —H —OMe —H —S(═O)₂iPr D55 —H —H —H —SMe —OMe —H —S(═O)₂iPr D56 —OCF₃ —H —H —H —OMe —H —S(═O)₂iPr D57 —H —OCF₃ —H —H —OMe —H —S(═O)₂iPr D58 —H —H —OCF₃ —H —OMe —H —S(═O)₂iPr D59 —H —H —H —OCF₃ —OMe —H —S(═O)₂iPr

TABLE 62

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) D60 —H —H —H —H —OMe —H —S(═O)₂Et D61 —H —H —H —H —OMe —H —S(═O)₂Me D62 —H —H —H —H —OMe —H —S(═O)₂NHMe D63 —H —H —H —H —OMe —H —S(═O)₂NMe₂ D64 —H —H —H —H —OMe —H —C(═O)NHMe D65 —H —H —H —H —OMe —H —C(═O)NMe₂ D66 —H —H —H —H —OMe —H —C(═O)iPr D67 —H —H —H —H —OMe —H —C(═O)Et D68 —H —H —H —H —OMe —H —F D69 —H —H —H —H —OMe —H —Cl D70 —H —H —H —H —OMe —H —Br D71 —H —H —H —H —OMe —H —OMe D72 —H —H —H —H —OMe —H —OEt D73 —H —H —H —H —OMe —H —OiPr D74 —H —H —H —H —OMe —H —OCF₃ D75 —H —H —H —H —OMe —H —SMe D76 —H —H —H —H —OMe —H —SEt D77 —H —H —H —H —OMe —H —SiPr D78 —H —H —H —H —OMe —H Me D79 —H —H —H —H —OMe —H Et D80 —H —H —H —H —OMe —H iPr D81 —H —H —H —H —OMe —H —CF₃

TABLE 63

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) E1  —H —H —H —H —F —H —S(═O)₂iPr E2  —H —H —H —H —Cl —H —S(═O)₂iPr E3  —H —H —H —H —Br —H —S(═O)₂iPr E4  —H —H —H —H —OEt —H —S(═O)₂iPr E5  —H —H —H —H —OiPr —H —S(═O)₂iPr E6  —H —H —H —H —CF₃ —H —S(═O)₂iPr E7  —H —H —H —H —CN —H —S(═O)₂iPr E8  —H —H —H —H Me —H —S(═O)₂iPr E9  —H —H —H —H Et —H —S(═O)₂iPr E10 —H —H —H —H —SMe —H —S(═O)₂iPr E11 —H —H —H —H —OCF₃ —H —S(═O)₂iPr E12 —H —H —H —H —OMe —Cl —S(═O)₂iPr E13 —H —H —H —H —OMe —Br —S(═O)₂iPr E14 —H —H —H —H —OMe Me —S(═O)₂iPr E15 —H —H —H —H —OMe —SMe —S(═O)₂iPr E16 —H —H —H —H —OMe —NMe₂ —S(═O)₂iPr E17 —H —H —H —H —OMe —NEt₂ —S(═O)₂iPr E18 —H —H —H —H —OMe

—S(═O)₂iPr E19 —H —H —H —H —OMe

—S(═O)₂iPr E20 —H —H —H —H —OMe

—S(═O)₂iPr E21 —H —H —H —H —OMe

—S(═O)₂iPr E22 —H —H —H —H —OMe —CN —S(═O)₂iPr E23 —F —H —H —H —OMe —H —S(═O)₂iPr E24 —H —F —H —H —OMe —H —S(═O)₂iPr E25 —H —H —F —H —OMe —H —S(═O)₂iPr E26 —H —H —H —F —OMe —H —S(═O)₂iPr

TABLE 64

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) E27 —Cl —H —H —H —OMe —H —S(═O)₂iPr E28 —H —Cl —H —H —OMe —H —S(═O)₂iPr E29 —H —H —Cl —H —OMe —H —S(═O)₂iPr E30 —H —H —H —Cl —OMe —H —S(═O)₂iPr E31 —Br —H —H —H —OMe —H —S(═O)₂iPr E32 —H —Br —H —H —OMe —H —S(═O)₂iPr E33 —H —H —Br —H —OMe —H —S(═O)₂iPr E34 —H —H —H —Br —OMe —H —S(═O)₂iPr E35 Me —H —H —H —OMe —H —S(═O)₂iPr E36 —H Me —H —H —OMe —H —S(═O)₂iPr E37 —H —H Me —H —OMe —H —S(═O)₂iPr E39 —H —H —H Me —OMe —H —S(═O)₂iPr E40 —OMe —H —H —H —OMe —H —S(═O)₂iPr E41 —H —OMe —H —H —OMe —H —S(═O)₂iPr E42 —H —H —OMe —H —OMe —H —S(═O)₂iPr E43 —H —H —H —OMe —OMe —H —S(═O)₂iPr E44 —CN —H —H —H —OMe —H —S(═O)₂iPr E45 —H —CN —H —H —OMe —H —S(═O)₂iPr E46 —H —H —CN —H —OMe —H —S(═O)₂iPr E47 —H —H —H —CN —OMe —H —S(═O)₂iPr E48 —CF₃ —H —H —H —OMe —H —S(═O)₂iPr E49 —H —CF₃ —H —H —OMe —H —S(═O)₂iPr E50 —H —H —CF₃ —H —OMe —H —S(═O)₂iPr E51 —H —H —H —CF₃ —OMe —H —S(═O)₂iPr E52 —SMe —H —H —H —OMe —H —S(═O)₂iPr E53 —H —SMe —H —H —OMe —H —S(═O)₂iPr E54 —H —H —SMe —H —OMe —H —S(═O)₂iPr E55 —H —H —H —SMe —OMe —H —S(═O)₂iPr E56 —OCF₃ —H —H —H —OMe —H —S(═O)₂iPr E57 —H —OCF₃ —H —H —OMe —H —S(═O)₂iPr E58 —H —H —OCF₃ —H —OMe —H —S(═O)₂iPr E59 —H —H —H —OCF₃ —OMe —H —S(═O)₂iPr

TABLE 65

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) —R^(A′) E60 —H —H —H —H —OMe —H —S(═O)₂Et E61 —H —H —H —H —OMe —H —S(═O)₂Me E62 —H —H —H —H —OMe —H —S(═O)₂NHMe E63 —H —H —H —H —OMe —H —S(═O)₂NMe₂ E64 —H —H —H —H —OMe —H —C(═O)NHMe E65 —H —H —H —H —OMe —H —C(═O)NMe₂ E66 —H —H —H —H —OMe —H —C(═O)iPr E67 —H —H —H —H —OMe —H —C(═O)Et E68 —H —H —H —H —OMe —H —F E69 —H —H —H —H —OMe —H —Cl E70 —H —H —H —H —OMe —H —Br E71 —H —H —H —H —OMe —H —OMe E72 —H —H —H —H —OMe —H —OEt E73 —H —H —H —H —OMe —H —OiPr E74 —H —H —H —H —OMe —H —OCF₃ E75 —H —H —H —H —OMe —H —SMe E76 —H —H —H —H —OMe —H —SEt E77 —H —H —H —H —OMe —H —SiPr E78 —H —H —H —H —OMe —H Me E79 —H —H —H —H —OMe —H Et E80 —H —H —H —H —OMe —H iPr E81 —H —H —H —H —OMe —H —CF₃

TABLE 66

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) F1  —H —H —H —H —F —H F2  —H —H —H —H —Cl —H F3  —H —H —H —H —Br —H F4  —H —H —H —H —OEt —H F5  —H —H —H —H —OiPr —H F6  —H —H —H —H —CF₃ —H F7  —H —H —H —H —CN —H F8  —H —H —H —H Me —H F9  —H —H —H —H Et —H F10 —H —H —H —H —SMe —H F11 —H —H —H —H —OCF₃ —H F12 —H —H —H —H —OMe —Cl F13 —H —H —H —H —OMe —Br F14 —H —H —H —H —OMe Me F15 —H —H —H —H —OMe —SMe F16 —H —H —H —H —OMe —NMe₂ F17 —H —H —H —H —OMe —NEt₂ F18 —H —H —H —H —OMe

F19 —H —H —H —H —OMe

F20 —H —H —H —H —OMe

F21 —H —H —H —H —OMe

F22 —H —H —H —H —OMe —CN F23 —F —H —H —H —OMe —H F24 —H —F —H —H —OMe —H F25 —H —H —F —H —OMe —H F26 —H —H —H —F —OMe —H F27 —Cl —H —H —H —OMe —H F28 —H —Cl —H —H —OMe —H

TABLE 67

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) F29 —H —H —Cl —H —OMe —H F30 —H —H —H —Cl —OMe —H F31 —Br —H —H —H —OMe —H F32 —H —Br —H —H —OMe —H F33 —H —H —Br —H —OMe —H F34 —H —H —H —Br —OMe —H F35 Me —H —H —H —OMe —H F36 —H Me —H —H —OMe —H F37 —H —H Me —H —OMe —H F39 —H —H —H Me —OMe —H F40 —OMe —H —H —H —OMe —H F41 —H —OMe —H —H —OMe —H F42 —H —H —OMe —H —OMe —H F43 —H —H —H —OMe —OMe —H F44 —CN —H —H —H —OMe —H F45 —H —CN —H —H —OMe —H F46 —H —H —CN —H —OMe —H F47 —H —H —H —CN —OMe —H F48 —CF₃ —H —H —H —OMe —H F49 —H —CF₃ —H —H —OMe —H F50 —H —H —CF₃ —H —OMe —H F51 —H —H —H —CF₃ —OMe —H F52 —SMe —H —H —H —OMe —H F53 —H —SMe —H —H —OMe —H F54 —H —H —SMe —H —OMe —H F55 —H —H —H —SMe —OMe —H F56 —OCF₃ —H —H —H —OMe —H F57 —H —OCF₃ —H —H —OMe —H F58 —H —H —OCF₃ —H —OMe —H F59 —H —H —H —OCF₃ —OMe —H

TABLE 68

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) G1  —H —H —H —H —F —H G2  —H —H —H —H —Cl —H G3  —H —H —H —H —Br —H G4  —H —H —H —H —OEt —H G5  —H —H —H —H —OiPr —H G6  —H —H —H —H —CF₃ —H G7  —H —H —H —H —CN —H G8  —H —H —H —H Me —H G9  —H —H —H —H Et —H G10 —H —H —H —H —SMe —H G11 —H —H —H —H —OCF₃ —H G12 —H —H —H —H —OMe —Cl G13 —H —H —H —H —OMe —Br G14 —H —H —H —H —OMe Me G15 —H —H —H —H —OMe —SMe G16 —H —H —H —H —OMe —NMe₂ G17 —H —H —H —H —OMe —NEt₂ G18 —H —H —H —H —OMe

G19 —H —H —H —H —OMe

G20 —H —H —H —H —OMe

G21 —H —H —H —H —OMe

G22 —H —H —H —H —OMe —CN G23 —F —H —H —H —OMe —H G24 —H —F —H —H —OMe —H G25 —H —H —F —H —OMe —H G26 —H —H —H —F —OMe —H

TABLE 69

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) G27 —Cl —H —H —H —OMe —H G28 —H —Cl —H —H —OMe —H G29 —H —H —Cl —H —OMe —H G30 —H —H —H —Cl —OMe —H G31 —Br —H —H —H —OMe —H G32 —H —Br —H —H —OMe —H G33 —H —H —Br —H —OMe —H G34 —H —H —H —Br —OMe —H G35 Me —H —H —H —OMe —H G36 —H Me —H —H —OMe —H G37 —H —H Me —H —OMe —H G39 —H —H —H Me —OMe —H G40 —OMe —H —H —H —OMe —H G41 —H —OMe —H —H —OMe —H G42 —H —H —OMe —H —OMe —H G43 —H —H —H —OMe —OMe —H G44 —CN —H —H —H —OMe —H G45 —H —CN —H —H —OMe —H G46 —H —H —CN —H —OMe —H G47 —H —H —H —CN —OMe —H G48 —CF₃ —H —H —H —OMe —H G49 —H —CF₃ —H —H —OMe —H G50 —H —H —CF₃ —H —OMe —H G51 —H —H —H —CF₃ —OMe —H G52 —SMe —H —H —H —OMe —H G53 —H —SMe —H —H —OMe —H G54 —H —H —SMe —H —OMe —H G55 —H —H —H —SMe —OMe —H G56 —OCF₃ —H —H —H —OMe —H G57 —H —OCF₃ —H —H —OMe —H G58 —H —H —OCF₃ —H —OMe —H G59 —H —H —H —OCF₃ —OMe —H

TABLE 70

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) H1  —H —H —H —H —F —H H2  —H —H —H —H —Cl —H H3  —H —H —H —H —Br —H H4  —H —H —H —H —OEt —H H5  —H —H —H —H —OiPr —H H6  —H —H —H —H —CF₃ —H H7  —H —H —H —H —CN —H H8  —H —H —H —H Me —H H9  —H —H —H —H Et —H H10 —H —H —H —H —SMe —H H11 —H —H —H —H —OCF₃ —H H12 —H —H —H —H —OMe —Cl H13 —H —H —H —H —OMe —Br H14 —H —H —H —H —OMe Me H15 —H —H —H —H —OMe —SMe H16 —H —H —H —H —OMe —NMe₂ H17 —H —H —H —H —OMe —NEt₂ H18 —H —H —H —H —OMe

H19 —H —H —H —H —OMe

H20 —H —H —H —H —OMe

H21 —H —H —H —H —OMe

H22 —H —H —H —H —OMe —CN H23 —F —H —H —H —OMe —H H24 —H —F —H —H —OMe —H H25 —H —H —F —H —OMe —H H26 —H —H —H —F —OMe —H

TABLE 71

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(5′) H27 —Cl —H —H —H —OMe —H H28 —H —Cl —H —H —OMe —H H29 —H —H —Cl —H —OMe —H H30 —H —H —H —Cl —OMe —H H31 —Br —H —H —H —OMe —H H32 —H —Br —H —H —OMe —H H33 —H —H —Br —H —OMe —H H34 —H —H —H —Br —OMe —H H35 Me —H —H —H —OMe —H H36 —H Me —H —H —OMe —H H37 —H —H Me —H —OMe —H H39 —H —H —H Me —OMe —H H40 —OMe —H —H —H —OMe —H H41 —H —OMe —H —H —OMe —H H42 —H —H —OMe —H —OMe —H H43 —H —H —H —OMe —OMe —H H44 —CN —H —H —H —OMe —H H45 —H —CN —H —H —OMe —H H46 —H —H —CN —H —OMe —H H47 —H —H —H —CN —OMe —H H48 —CF₃ —H —H —H —OMe —H H49 —H —CF₃ —H —H —OMe —H H50 —H —H —CF₃ —H —OMe —H H51 —H —H —H —CF₃ —OMe —H H52 —SMe —H —H —H —OMe —H H53 —H —SMe —H —H —OMe —H H54 —H —H —SMe —H —OMe —H H55 —H —H —H —SMe —OMe —H H56 —OCF₃ —H —H —H —OMe —H H57 —H —OCF₃ —H —H —OMe —H H58 —H —H —OCF₃ —H —OMe —H H59 —H —H —H —OCF₃ —OMe —H

TABLE 72

No —R^(3′) I1 

I2 

I3 

I4 

I5 

I6 

I7 

I8 

I9 

I10

I11

I12

I13

I14

I15

I16

I17

I18

I19

I20

I21

I22

I23

I24

TABLE 73

No —R^(3′) I25

I26

I27

I28

I29

I30

I31

I32

I33

I34

I35

I36

I37

I38

I39

I40

I41

I42

I43

I44

I45

I46

I47

I48

TABLE 74

No —R^(3′) I49

I50

I51

I52

I53

I54

I55

I56

I57

I58

I59

I60

I61

I62

I63

I64

I65

I66

I67

I68

I69

I70

I71

I72

TABLE 75

No —R^(4′) J1 

J2 

J3 

J4 

J5 

J6 

J7 

J8 

J9 

J10

J11

J12

J13

J14

J15

J16

J17

J18

J19

J20

J21

J22

J23

J24

TABLE 76

No —R^(4′) J25

J26

J27

J28

J29

J30

J31

J32

J33

J34

J35

J36

J37

J38

J39

J40

J41

J42

J43

J44

J45

J46

J47

J48

TABLE 77

No —R^(4′) J49

J50

J51

J52

J53

J54

J55

J56

J57

J58

J59

J60

J61

J62

J63

J64

J65

J66

J67

J68

J69

J70

J71

J72

TABLE 78

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) K1  —H —H —H —H —F —S(═O)₂iPr K2  —H —H —H —H —Cl —S(═O)₂iPr K3  —H —H —H —H —Br —S(═O)₂iPr K4  —H —H —H —H —OEt —S(═O)₂iPr K5  —H —H —H —H —OiPr —S(═O)₂iPr K6  —H —H —H —H —CF₃ —S(═O)₂iPr K7  —H —H —H —H —CN —S(═O)₂iPr K8  —H —H —H —H Me —S(═O)₂iPr K9  —H —H —H —H Et —S(═O)₂iPr K10 —H —H —H —H —SMe —S(═O)₂iPr K11 —H —H —H —H —OCF₃ —S(═O)₂iPr K12 —F —H —H —H —OMe —S(═O)₂iPr K13 —H —F —H —H —OMe —S(═O)₂iPr K14 —H —H —F —H —OMe —S(═O)₂iPr K15 —H —H —H —F —OMe —S(═O)₂iPr K16 —Cl —H —H —H —OMe —S(═O)₂iPr K17 —H —Cl —H —H —OMe —S(═O)₂iPr K18 —H —H —Cl —H —OMe —S(═O)₂iPr K19 —H —H —H —Cl —OMe —S(═O)₂iPr K20 —Br —H —H —H —OMe —S(═O)₂iPr K21 —H —Br —H —H —OMe —S(═O)₂iPr K22 —H —H —Br —H —OMe —S(═O)₂iPr K23 —H —H —H —Br —OMe —S(═O)₂iPr K24 Me —H —H —H —OMe —S(═O)₂iPr K25 —H Me —H —H —OMe —S(═O)₂iPr K26 —H —H Me —H —OMe —S(═O)₂iPr K27 —H —H —H Me —OMe —S(═O)₂iPr K28 —OMe —H —H —H —OMe —S(═O)₂iPr K29 —H —OMe —H —H —OMe —S(═O)₂iPr K30 —H —H —OMe —H —OMe —S(═O)₂iPr K31 —H —H —H —OMe —OMe —S(═O)₂iPr K32 —CN —H —H —H —OMe —S(═O)₂iPr K33 —H —CN —H —H —OMe —S(═O)₂iPr K34 —H —H —CN —H —OMe —S(═O)₂iPr K35 —H —H —H —CN —OMe —S(═O)₂iPr

TABLE 79

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) K36 —CF₃ —H —H —H —OMe —S(═O)₂iPr K37 —H —CF₃ —H —H —OMe —S(═O)₂iPr K38 —H —H —CF₃ —H —OMe —S(═O)₂iPr K39 —H —H —H —CF₃ —OMe —S(═O)₂iPr K40 —SMe —H —H —H —OMe —S(═O)₂iPr K41 —H —SMe —H —H —OMe —S(═O)₂iPr K42 —H —H —SMe —H —OMe —S(═O)₂iPr K43 —H —H —H —SMe —OMe —S(═O)₂iPr K44 —OCF₃ —H —H —H —OMe —S(═O)₂iPr K45 —H —OCF₃ —H —H —OMe —S(═O)₂iPr K46 —H —H —OCF₃ —H —OMe —S(═O)₂iPr K47 —H —H —H —OCF₃ —OMe —S(═O)₂iPr K48 —H —H —H —H —OMe —S(═O)₂Et K49 —H —H —H —H —OMe —S(═O)₂Me K50 —H —H —H —H —OMe —S(═O)₂NHMe K51 —H —H —H —H —OMe —S(═O)₂NMe₂ K52 —H —H —H —H —OMe —C(═O)NHMe K53 —H —H —H —H —OMe —C(═O)NMe₂ K54 —H —H —H —H —OMe —C(═O)iPr K55 —H —H —H —H —OMe —C(═O)Et K56 —H —H —H —H —OMe —F K57 —H —H —H —H —OMe —Cl K58 —H —H —H —H —OMe —Br K59 —H —H —H —H —OMe —OMe K60 —H —H —H —H —OMe —OEt K61 —H —H —H —H —OMe —OiPr K62 —H —H —H —H —OMe —OCF₃ K63 —H —H —H —H —OMe —SMe K64 —H —H —H —H —OMe —SEt K65 —H —H —H —H —OMe —SiPr K66 —H —H —H —H —OMe Me K67 —H —H —H —H —OMe Et K68 —H —H —H —H —OMe iPr K69 —H —H —H —H —OMe —CF₃

TABLE 80

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) L1  —H —H —H —H —F —S(═O)₂iPr L2  —H —H —H —H —Cl —S(═O)₂iPr L3  —H —H —H —H —Br —S(═O)₂iPr L4  —H —H —H —H —OEt —S(═O)₂iPr L5  —H —H —H —H —OiPr —S(═O)₂iPr L6  —H —H —H —H —CF₃ —S(═O)₂iPr L7  —H —H —H —H —CN —S(═O)₂iPr L8  —H —H —H —H Me —S(═O)₂iPr L9  —H —H —H —H Et —S(═O)₂iPr L10 —H —H —H —H —SMe —S(═O)₂iPr L11 —H —H —H —H —OCF₃ —S(═O)₂iPr L12 —F —H —H —H —OMe —S(═O)₂iPr L13 —H —F —H —H —OMe —S(═O)₂iPr L14 —H —H —F —H —OMe —S(═O)₂iPr L15 —H —H —H —F —OMe —S(═O)₂iPr L16 —Cl —H —H —H —OMe —S(═O)₂iPr L17 —H —Cl —H —H —OMe —S(═O)₂iPr L18 —H —H —Cl —H —OMe —S(═O)₂iPr L19 —H —H —H —Cl —OMe —S(═O)₂iPr L20 —Br —H —H —H —OMe —S(═O)₂iPr L21 —H —Br —H —H —OMe —S(═O)₂iPr L22 —H —H —Br —H —OMe —S(═O)₂iPr L23 —H —H —H —Br —OMe —S(═O)₂iPr L24 Me —H —H —H —OMe —S(═O)₂iPr L25 —H Me —H —H —OMe —S(═O)₂iPr L26 —H —H Me —H —OMe —S(═O)₂iPr L27 —H —H —H Me —OMe —S(═O)₂iPr L28 —OMe —H —H —H —OMe —S(═O)₂iPr L29 —H —OMe —H —H —OMe —S(═O)₂iPr L30 —H —H —OMe —H —OMe —S(═O)₂iPr L31 —H —H —H —OMe —OMe —S(═O)₂iPr L32 —CN —H —H —H —OMe —S(═O)₂iPr L33 —H —CN —H —H —OMe —S(═O)₂iPr L34 —H —H —CN —H —OMe —S(═O)₂iPr L35 —H —H —H —CN —OMe —S(═O)₂iPr

TABLE 81

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) L36 —CF₃ —H —H —H —OMe —S(═O)₂iPr L37 —H —CF₃ —H —H —OMe —S(═O)₂iPr L38 —H —H —CF₃ —H —OMe —S(═O)₂iPr L39 —H —H —H —CF₃ —OMe —S(═O)₂iPr L40 —SMe —H —H —H —OMe —S(═O)₂iPr L41 —H —SMe —H —H —OMe —S(═O)₂iPr L42 —H —H —SMe —H —OMe —S(═O)₂iPr L43 —H —H —H —SMe —OMe —S(═O)₂iPr L44 —OCF₃ —H —H —H —OMe —S(═O)₂iPr L45 —H —OCF₃ —H —H —OMe —S(═O)₂iPr L46 —H —H —OCF₃ —H —OMe —S(═O)₂iPr L47 —H —H —H —OCF₃ —OMe —S(═O)₂iPr L48 —H —H —H —H —OMe —S(═O)₂Et L49 —H —H —H —H —OMe —S(═O)₂Me L50 —H —H —H —H —OMe —S(═O)₂NHMe L51 —H —H —H —H —OMe —S(═O)₂NMe₂ L52 —H —H —H —H —OMe —C(═O)NHMe L53 —H —H —H —H —OMe —C(═O)NMe₂ L54 —H —H —H —H —OMe —C(═O)iPr L55 —H —H —H —H —OMe —C(═O)Et L56 —H —H —H —H —OMe —F L57 —H —H —H —H —OMe —Cl L58 —H —H —H —H —OMe —Br L59 —H —H —H —H —OMe —OMe L60 —H —H —H —H —OMe —OEt L61 —H —H —H —H —OMe —OiPr L62 —H —H —H —H —OMe —OCF₃ L63 —H —H —H —H —OMe —SMe L64 —H —H —H —H —OMe —SEt L65 —H —H —H —H —OMe —SiPr L66 —H —H —H —H —OMe Me L67 —H —H —H —H —OMe Et L68 —H —H —H —H —OMe iPr L69 —H —H —H —H —OMe —CF₃

TABLE 82

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) M1 —H —H —H —H —F —S(═O)₂iPr M2 —H —H —H —H —Cl —S(═O)₂iPr M3 —H —H —H —H —Br —S(═O)₂iPr M4 —H —H —H —H —OEt —S(═O)₂iPr M5 —H —H —H —H —OiPr —S(═O)₂iPr M6 —H —H —H —H —CF₃ —S(═O)₂iPr M7 —H —H —H —H —CN —S(═O)₂iPr M8 —H —H —H —H Me —S(═O)₂iPr M9 —H —H —H —H Et —S(═O)₂iPr M10 —H —H —H —H —SMe —S(═O)₂iPr M11 —H —H —H —H —OCF₃ —S(═O)₂iPr M12 —F —H —H —H —OMe —S(═O)₂iPr M13 —H —F —H —H —OMe —S(═O)₂Pr M14 —H —H —F —H —OMe —S(═O)₂Pr M15 —H —H —H —F —OMe —S(═O)₂iPr M16 —Cl —H —H —H —OMe —S(═O)₂iPr M17 —H —Cl —H —H —OMe —S(═O)₂iPr M18 —H —H —Cl —H —OMe —S(═O)₂iPr M19 —H —H —H —Cl —OMe —S(═O)₂iPr M20 —Br —H —H —H —OMe —S(═O)₂iPr M21 —H —Br —H —H —OMe —S(═O)₂iPr M22 —H —H —Br —H —OMe —S(═O)₂iPr M23 —H —H —H —Br —OMe —S(═O)₂iPr M24 Me —H —H —H —OMe —S(═O)₂iPr M25 —H Me —H —H —OMe —S(═O)₂iPr M26 —H —H Me —H —OMe —S(═O)₂iPr M27 —H —H —H Me —OMe —S(═O)₂iPr M28 —OMe —H —H —H —OMe —S(═O)₂iPr M29 —H —OMe —H —H —OMe —S(═O)₂iPr M30 —H —H —OMe —H —OMe —S(═O)₂iPr M31 —H —H —H —OMe —OMe —S(═O)₂iPr M32 —CN —H —H —H —OMe —S(═O)2iPr M33 —H —CN —H —H —OMe —S(═O)2iPr M34 —H —H —CN —H —OMe —S(═O)2iPr M35 —H —H —H —CN —OMe —S(═O)2iPr

TABLE 83

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) —R^(A′) M36 —CF₃ —H —H —H —OMe —S(═O)₂iPr M37 —H —CF₃ —H —H —OMe —S(═O)₂iPr M38 —H —H —CF₃ —H —OMe —S(═O)₂iPr M39 —H —H —H —CF₃ —OMe —S(═O)₂iPr M40 —SMe —H —H —H —OMe —S(═O)₂iPr M41 —H —SMe —H —H —OMe —S(═O)₂iPr M42 —H —H —SMe —H —OMe —S(═O)₂iPr M43 —H —H —H —SMe —OMe —S(═O)₂iPr M44 —OCF₃ —H —H —H —OMe —S(═O)₂iPr M45 —H —OCF₃ —H —H —OMe —S(═O)₂iPr M46 —H —H —OCF₃ —H —OMe —S(═O)₂iPr M47 —H —H —H —OCF₃ —OMe —S(═O)₂iPr M48 —H —H —H —H —OMe —S(═O)₂Et M49 —H —H —H —H —OMe —S(═O)₂Me M50 —H —H —H —H —OMe —S(═O)₂NHMe M51 —H —H —H —H —OMe —S(═O)₂NMe₂ M52 —H —H —H —H —OMe —C(═O)NHMe M53 —H —H —H —H —OMe —C(═O)NMe₂ M54 —H —H —H —H —OMe —C(═O)iPr M55 —H —H —H —H —OMe —C(═O)Et M56 —H —H —H —H —OMe —F M57 —H —H —H —H —OMe —Cl M58 —H —H —H —H —OMe —Br M59 —H —H —H —H —OMe —OMe M60 —H —H —H —H —OMe —OEt M61 —H —H —H —H —OMe —OiPr M62 —H —H —H —H —OMe —OCF₃ M63 —H —H —H —H —OMe —SMe M64 —H —H —H —H —OMe —SEt M65 —H —H —H —H —OMe —SiPr M66 —H —H —H —H —OMe Me M67 —H —H —H —H —OMe Et M68 —H —H —H —H —OMe iPr M69 —H —H —H —H —OMe —CF₃

TABLE 84

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) N1 —H —H —H —H —F N2 —H —H —H —H —Cl N3 —H —H —H —H —Br N4 —H —H —H —H —OEt N5 —H —H —H —H —OiPr N6 —H —H —H —H —CF₃ N7 —H —H —H —H —CN N8 —H —H —H —H Me N9 —H —H —H —H Et N10 —H —H —H —H —SMe N11 —H —H —H —H —OCF₃ N12 —F —H —H —H —OMe N13 —H —F —H —H —OMe N14 —H —H —F —H —OMe N15 —H —H —H —F —OMe N16 —Cl —H —H —H —OMe N17 —H —Cl —H —H —OMe N18 —H —H —Cl —H —OMe N19 —H —H —H —Cl —OMe N20 —Br —H —H —H —OMe N21 —H —Br —H —H —OMe N22 —H —H —Br —H —OMe N23 —H —H —H —Br —OMe N24 Me —H —H —H —OMe N25 —H Me —H —H —OMe N26 —H —H Me —H —OMe N27 —H —H —H Me —OMe

TABLE 85

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) N28 —OMe —H —H —H —OMe N29 —H —OMe —H —H —OMe N30 —H —H —OMe —H —OMe N31 —H —H —H —OMe —OMe N32 —CN —H —H —H —OMe N33 —H —CN —H —H —OMe N34 —H —H —CN —H —OMe N35 —H —H —H —CN —OMe N36 —CF₃ —H —H —H —OMe N37 —H —CF₃ —H —H —OMe N38 —H —H —CF₃ —H —OMe N39 —H —H —H —CF₃ —OMe N40 —SMe —H —H —H —OMe N41 —H —SMe —H —H —OMe N42 —H —H —SMe —H —OMe N43 —H —H —H —SMe —OMe N44 —OCF₃ —H —H —H —OMe N45 —H —OCF₃ —H —H —OMe N46 —H —H —OCF₃ —H —OMe N47 —H —H —H —OCF₃ —OMe

TABLE 86

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) O1 —H —H —H —H —F O2 —H —H —H —H —Cl O3 —H —H —H —H —Br O4 —H —H —H —H —OEt O5 —H —H —H —H —OiPr O6 —H —H —H —H —CF₃ O7 —H —H —H —H —CN O8 —H —H —H —H Me O9 —H —H —H —H Et O10 —H —H —H —H —SMe O11 —H —H —H —H —OCF₃ O12 —F —H —H —H —OMe O13 —H —F —H —H —OMe O14 —H —H —F —H —OMe O15 —H —H —H —F —OMe O16 —Cl —H —H —H —OMe O17 —H —Cl —H —H —OMe O18 —H —H —Cl —H —OMe O19 —H —H —H —Cl —OMe O20 —Br —H —H —H —OMe O21 —H —Br —H —H —OMe O22 —H —H —Br —H —OMe O23 —H —H —H —Br —OMe O24 Me —H —H —H —OMe O25 —H Me —H —H —OMe O26 —H —H Me —H —OMe O27 —H —H —H Me —OMe

TABLE 87

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) O28 —OMe —H —H —H —OMe O29 —H —OMe —H —H —OMe O30 —H —H —OMe —H —OMe O31 —H —H —H —OMe —OMe O32 —CN —H —H —H —OMe O33 —H —CN —H —H —OMe O34 —H —H —CN —H —OMe O35 —H —H —H —CN —OMe O36 —CF₃ —H —H —H —OMe O37 —H —CF₃ —H —H —OMe O38 —H —H —CF₃ —H —OMe O39 —H —H —H —CF₃ —OMe O40 —SMe —H —H —H —OMe O41 —H —SMe —H —H —OMe O42 —H —H —SMe —H —OMe O43 —H —H —H —SMe —OMe O44 —OCF₃ —H —H —H —OMe O45 —H —OCF₃ —H —H —OMe O46 —H —H —OCF₃ —H —OMe O47 —H —H —H —OCF₃ —OMe

TABLE 88

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) P1 —H —H —H —H —F P2 —H —H —H —H —Cl P3 —H —H —H —H —Br P4 —H —H —H —H —OEt P5 —H —H —H —H —OiPr P6 —H —H —H —H —CF₃ P7 —H —H —H —H —CN P8 —H —H —H —H Me P9 —H —H —H —H Et P10 —H —H —H —H —SMe P11 —H —H —H —H —OCF₃ P12 —F —H —H —H —OMe P13 —H —F —H —H —OMe P14 —H —H —F —H —OMe P15 —H —H —H —F —OMe P16 —Cl —H —H —H —OMe P17 —H —Cl —H —H —OMe P18 —H —H —Cl —H —OMe P19 —H —H —H —Cl —OMe P20 —Br —H —H —H —OMe P21 —H —Br —H —H —OMe P22 —H —H —Br —H —OMe P23 —H —H —H —Br —OMe P24 Me —H —H —H —OMe P25 —H Me —H —H —OMe P26 —H —H Me —H —OMe P27 —H —H —H Me —OMe

TABLE 89

No —R^(1a′) —R^(1b′) —R^(1c′) —R^(1d′) —R^(2′) P28 —OMe —H —H —H —OMe P29 —H —OMe —H —H —OMe P30 —H —H —OMe —H —OMe P31 —H —H —H —OMe —OMe P32 —CN —H —H —H —OMe P33 —H —CN —H —H —OMe P34 —H —H —CN —H —OMe P35 —H —H —H —CN —OMe P36 —CF₃ —H —H —H —OMe P37 —H —CF₃ —H —H —OMe P38 —H —H —CF₃ —H —OMe P39 —H —H —H —CF₃ —OMe P40 —SMe —H —H —H —OMe P41 —H —SMe —H —H —OMe P42 —H —H —SMe —H —OMe P43 —H —H —H —SMe —OMe P44 —OCF₃ —H —H —H —OMe P45 —H —OCF₃ —H —H —OMe P46 —H —H —OCF₃ —H —OMe P47 —H —H —H —OCF₃ —OMe

TABLE 90

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) Q1 —F —H —H —H —S(═O)₂iPr Q2 —H —F —H —H —S(═O)₂iPr Q3 —H —H —F —H —S(═O)₂iPr Q4 —H —H —H —F —S(═O)₂iPr Q5 —Cl —H —H —H —S(═O)₂iPr Q6 —H —Cl —H —H —S(═O)₂iPr Q7 —H —H —Cl —H —S(═O)₂iPr Q8 —H —H —H —Cl —S(═O)₂iPr Q9 —Br —H —H —H —S(═O)₂iPr Q10 —H —Br —H —H —S(═O)₂iPr Q11 —H —H —Br —H —S(═O)₂iPr Q12 —H —H —H —Br —S(═O)₂iPr Q13 Me —H —H —H —S(═O)₂Pr Q14 —H Me —H —H —S(═O)₂Pr Q15 —H —H Me —H —S(═O)₂iPr Q16 —H —H —H Me —S(═O)₂iPr Q17 —OMe —H —H —H —S(═O)₂iPr Q18 —H —OMe —H —H —S(═O)₂iPr Q19 —H —H —OMe —H —S(═O)₂iPr Q20 —H —H —H —OMe —S(═O)₂iPr Q21 —CN —H —H —H —S(═O)₂iPr Q22 —H —CN —H —H —S(═O)₂iPr Q23 —H —H —CN —H —S(═O)₂iPr Q24 —H —H —H —CN —S(═O)₂iPr Q25 —CF₃ —H —H —H —S(═O)₂iPr Q26 —H —CF₃ —H —H —S(═O)₂iPr Q27 —H —H —CF₃ —H —S(═O)₂iPr Q28 —H —H —H —CF₃ —S(═O)₂iPr Q29 —SMe —H —H —H —S(═O)₂iPr Q30 —H —SMe —H —H —S(═O)₂iPr Q31 —H —H —SMe —H —S(═O)₂iPr Q32 —H —H —H —SMe —S(═O)₂iPr Q33 —OCF₃ —H —H —H —S(═O)₂iPr Q34 —H —OCF₃ —H —H —S(═O)₂iPr Q35 —H —H —OCF₃ —H —S(═O)₂iPr Q36 —H —H —H —OCF₃ —S(═O)₂iPr

TABLE 91

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) Q37 —F —H —H —H —C(═O)NHMe Q38 —H —F —H —H —C(═O)NHMe Q39 —H —H —F —H —C(═O)NHMe Q40 —H —H —H —F —C(═O)NHMe Q41 —Cl —H —H —H —C(═O)NHMe Q42 —H —Cl —H —H —C(═O)NHMe Q43 —H —H —Cl —H —C(═O)NHMe Q44 —H —H —H —Cl —C(═O)NHMe Q45 —Br —H —H —H —C(═O)NHMe Q46 —H —Br —H —H —C(═O)NHMe Q47 —H —H —Br —H —C(═O)NHMe Q48 —H —H —H —Br —C(═O)NHMe Q49 Me —H —H —H —C(═O)NHMe Q50 —H Me —H —H —C(═O)NHMe Q51 —H —H Me —H —C(═O)NHMe Q52 —H —H —H Me —C(═O)NHMe Q53 —OMe —H —H —H —C(═O)NHMe Q54 —H —OMe —H —H —C(═O)NHMe Q55 —H —H —OMe —H —C(═O)NHMe Q56 —H —H —H —OMe —C(═O)NHMe Q57 —CN —H —H —H —C(═O)NHMe Q58 —H —CN —H —H —C(═O)NHMe Q59 —H —H —CN —H —C(═O)NHMe Q60 —H —H —H —CN —C(═O)NHMe Q61 —CF₃ —H —H —H —C(═O)NHMe Q62 —H —CF₃ —H —H —C(═O)NHMe Q63 —H —H —CF₃ —H —C(═O)NHMe Q64 —H —H —H —CF₃ —C(═O)NHMe Q65 —SMe —H —H —H —C(═O)NHMe Q66 —H —SMe —H —H —C(═O)NHMe Q67 —H —H —SMe —H —C(═O)NHMe Q68 —H —H —H —SMe —C(═O)NHMe Q69 —OCF₃ —H —H —H —C(═O)NHMe Q70 —H —OCF₃ —H —H —C(═O)NHMe Q71 —H —H —OCF₃ —H —C(═O)NHMe Q72 —H —H —H —OCF₃ —C(═O)NHMe

TABLE 92

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) R1 —F —H —H —H —S(═O)₂iPr R2 —H —F —H —H —S(═O)₂iPr R3 —H —H —F —H —S(═O)₂iPr R4 —H —H —H —F —S(═O)₂iPr R5 —Cl —H —H —H —S(═O)₂iPr R6 —H —Cl —H —H —S(═O)₂iPr R7 —H —H —Cl —H —S(═O)₂iPr R8 —H —H —H —Cl —S(═O)₂iPr R9 —Br —H —H —H —S(═O)₂iPr R10 —H —Br —H —H —S(═O)₂iPr R11 —H —H —Br —H —S(═O)₂iPr R12 —H —H —H —Br —S(═O)₂iPr R13 Me —H —H —H —S(═O)₂Pr R14 —H Me —H —H —S(═O)₂Pr R15 —H —H Me —H —S(═O)₂iPr R16 —H —H —H Me —S(═O)₂iPr R17 —OMe —H —H —H —S(═O)₂iPr R18 —H —OMe —H —H —S(═O)₂iPr R19 —H —H —OMe —H —S(═O)₂iPr R20 —H —H —H —OMe —S(═O)₂iPr R21 —CN —H —H —H —S(═O)₂iPr R22 —H —CN —H —H —S(═O)₂iPr R23 —H —H —CN —H —S(═O)₂iPr R24 —H —H —H —CN —S(═O)₂iPr R25 —CF₃ —H —H —H —S(═O)₂iPr R26 —H —CF₃ —H —H —S(═O)₂iPr R27 —H —H —CF₃ —H —S(═O)₂iPr R28 —H —H —H —CF₃ —S(═O)₂iPr R29 —SMe —H —H —H —S(═O)₂iPr R30 —H —SMe —H —H —S(═O)₂iPr R31 —H —H —SMe —H —S(═O)₂iPr R32 —H —H —H —SMe —S(═O)₂iPr R33 —OCF₃ —H —H —H —S(═O)2iPr R34 —H —OCF₃ —H —H —S(═O)2iPr R35 —H —H —OCF3 —H —S(═O)2iPr R36 —H —H —H —OCF3 —S(═O)2iPr

TABLE 93

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) R37 —F —H —H —H —C(═O)NHMe R38 —H —F —H —H —C(═O)NHMe R39 —H —H —F —H —C(═O)NHMe R40 —H —H —H —F —C(═O)NHMe R41 —Cl —H —H —H —C(═O)NHMe R42 —H —Cl —H —H —C(═O)NHMe R43 —H —H —Cl —H —C(═O)NHMe R44 —H —H —H —Cl —C(═O)NHMe R45 —Br —H —H —H —C(═O)NHMe R46 —H —Br —H —H —C(═O)NHMe R47 —H —H —Br —H —C(═O)NHMe R48 —H —H —H —Br —C(═O)NHMe R49 Me —H —H —H —C(═O)NHMe R50 —H Me —H —H —C(═O)NHMe R51 —H —H Me —H —C(═O)NHMe R52 —H —H —H Me —C(═O)NHMe R53 —OMe —H —H —H —C(═O)NHMe R54 —H —OMe —H —H —C(═O)NHMe R55 —H —H —OMe —H —C(═O)NHMe R56 —H —H —H —OMe —C(═O)NHMe R57 —CN —H —H —H —C(═O)NHMe R58 —H —CN —H —H —C(═O)NHMe R59 —H —H —CN —H —C(═O)NHMe R60 —H —H —H —CN —C(═O)NHMe R61 —CF₃ —H —H —H —C(═O)NHMe R62 —H —CF₃ —H —H —C(═O)NHMe R63 —H —H —CF₃ —H —C(═O)NHMe R64 —H —H —H —CF₃ —C(═O)NHMe R65 —SMe —H —H —H —C(═O)NHMe R66 —H —SMe —H —H —C(═O)NHMe R67 —H —H —SMe —H —C(═O)NHMe R68 —H —H —H —SMe —C(═O)NHMe R69 —OCF₃ —H —H —H —C(═O)NHMe R70 —H —OCF₃ —H —H —C(═O)NHMe R71 —H —H —OCF₃ —H —C(═O)NHMe R72 —H —H —H —OCF₃ —C(═O)NHMe

TABLE 94

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) S1 —F —H —H —H —S(═O)₂iPr S2 —H —F —H —H —S(═O)₂iPr S3 —H —H —F —H —S(═O)₂iPr S4 —H —H —H —F —S(═O)₂iPr S5 —Cl —H —H —H —S(═O)₂iPr S6 —H —Cl —H —H —S(═O)₂iPr S7 —H —H —Cl —H —S(═O)₂iPr S8 —H —H —H —Cl —S(═O)₂iPr S9 —Br —H —H —H —S(═O)₂iPr S10 —H —Br —H —H —S(═O)₂iPr S11 —H —H —Br —H —S(═O)₂iPr S12 —H —H —H —Br —S(═O)₂iPr S13 Me —H —H —H —S(═O)₂Pr S14 —H Me —H —H —S(═O)₂Pr S15 —H —H Me —H —S(═O)₂iPr S16 —H —H —H Me —S(═O)₂iPr S17 —OMe —H —H —H —S(═O)₂iPr S18 —H —OMe —H —H —S(═O)₂iPr S19 —H —H —OMe —H —S(═O)₂iPr S20 —H —H —H —OMe —S(═O)₂iPr S21 —CN —H —H —H —S(═O)₂iPr S22 —H —CN —H —H —S(═O)₂iPr S23 —H —H —CN —H —S(═O)₂iPr S24 —H —H —H —CN —S(═O)₂iPr S25 —CF₃ —H —H —H —S(═O)₂iPr S26 —H —CF₃ —H —H —S(═O)₂iPr S27 —H —H —CF₃ —H —S(═O)₂iPr S28 —H —H —H —CF₃ —S(═O)₂iPr S29 —SMe —H —H —H —S(═O)₂iPr S30 —H —SMe —H —H —S(═O)₂iPr S31 —H —H —SMe —H —S(═O)₂iPr S32 —H —H —H —SMe —S(═O)₂iPr S33 —OCF₃ —H —H —H —S(═O)2iPr S34 —H —OCF₃ —H —H —S(═O)2iPr S35 —H —H —OCF3 —H —S(═O)2iPr S36 —H —H —H —OCF3 —S(═O)2iPr

TABLE 95

No —R^(6a′) —R^(6b′) —R^(6c′) —R^(6d′) —R^(A′) S37 —F —H —H —H —C(═O)NHMe S38 —H —F —H —H —C(═O)NHMe S39 —H —H —F —H —C(═O)NHMe S40 —H —H —H —F —C(═O)NHMe S41 —Cl —H —H —H —C(═O)NHMe S42 —H —Cl —H —H —C(═O)NHMe S43 —H —H —Cl —H —C(═O)NHMe S44 —H —H —H —Cl —C(═O)NHMe S45 —Br —H —H —H —C(═O)NHMe S46 —H —Br —H —H —C(═O)NHMe S47 —H —H —Br —H —C(═O)NHMe S48 —H —H —H —Br —C(═O)NHMe S49 Me —H —H —H —C(═O)NHMe S50 —H Me —H —H —C(═O)NHMe S51 —H —H Me —H —C(═O)NHMe S52 —H —H —H Me —C(═O)NHMe S53 —OMe —H —H —H —C(═O)NHMe S54 —H —OMe —H —H —C(═O)NHMe S55 —H —H —OMe —H —C(═O)NHMe S56 —H —H —H —OMe —C(═O)NHMe S57 —CN —H —H —H —C(═O)NHMe S58 —H —CN —H —H —C(═O)NHMe S59 —H —H —CN —H —C(═O)NHMe S60 —H —H —H —CN —C(═O)NHMe S61 —CF₃ —H —H —H —C(═O)NHMe S62 —H —CF₃ —H —H —C(═O)NHMe S63 —H —H —CF₃ —H —C(═O)NHMe S64 —H —H —H —CF₃ —C(═O)NHMe S65 —SMe —H —H —H —C(═O)NHMe S66 —H —SMe —H —H —C(═O)NHMe S67 —H —H —SMe —H —C(═O)NHMe S68 —H —H —H —SMe —C(═O)NHMe S69 —OCF₃ —H —H —H —C(═O)NHMe S70 —H —OCF₃ —H —H —C(═O)NHMe S71 —H —H —OCF₃ —H —C(═O)NHMe S72 —H —H —H —OCF₃ —C(═O)NHMe

INDUSTRIAL APPLICABILITY

The compounds of formula (I) or salts thereof have inhibitory activity against the kinase activity of EML4-ALK fusion proteins and mutant EGFR proteins, as well as growth inhibitory activity against human non-small cell lung cancer cell lines NCI-H2228 and HCC827, and can be used as active ingredients in pharmaceutical compositions for preventing and/or treating cancer, such as lung cancer in one embodiment, non-small cell lung cancer or small cell lung cancer in another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer in yet another embodiment, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer in yet another embodiment, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer in yet another embodiment.

SEQUENCE LISTING FREE TEXT

The numerical heading <223> in the Sequence Listing shown below contains an explanation of “Artificial Sequence.” More specifically, each nucleotide sequence represented by the sequence of SEQ ID NO: 9 or 10 in the Sequence Listing is an artificially synthesized primer sequence. 

1-18. (canceled)
 19. A method for preventing or treating cancer, lung cancer, non-small cell lung cancer, small cell lung cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive cancer, EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive lung cancer, or EML4-ALK fusion polynucleotide-positive and/or mutant EGFR polynucleotide-positive non-small cell lung cancer, which comprises administering an effective amount of a compound of formula (I) or a salt thereof to a patient:

wherein: —X— represents (1) a group of formula (II), or (2) a group of formula (III)

—R⁵ represents (1) —H, (2) —OH, (3) halogen, (4) lower alkyl which may be substituted with one or more halogens, (5) O-lower alkyl which may be substituted with one or more halogens, (6) —S-lower alkyl, (7) cyano, (8) amino which may be substituted with one or two lower alkyls, or (9) cyclic amino which may be substituted with one or more groups selected from the group consisting of lower alkyl, oxo, —OH, —O-lower alkyl, and amino which may be substituted with one or two lower alkyls (provided that the triazine ring to which —R⁵ is attached is attached to the nitrogen atom in the cyclic amino), —R^(6a), —R^(6b), —R^(6c) and —R^(6d), K which may be the same or different, each represent (1) —H, (2) halogen, (3) lower alkyl which may be substituted with one or more halogens, (4) O-lower alkyl which may be substituted with one or more halogens, (5) —S-lower alkyl, or (6) cyano, —W represents a group represented by -A-B, -A- is —S(═O)₂—, -B is isopropyl, —R^(1a), —R^(1b), —R^(1c) and —R^(1d) are each —H, —R² is —O-methyl, —R⁴ is —H, and —R³ is 4-(4-methylpiperazin-1-yl)piperidin-1-yl.
 20. The method according to claim 1, wherein —X— is a group represented by formula (II) and —R⁵ is —H.
 21. The method according to claim 1, wherein said compound is N⁴-[2-(isopropylsulfonyl)phenyl]-N²-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}quinazoline-2,4-diamine.
 22. The method according to claim 1, wherein said compound is N-[2-(isopropylsulfonyl)phenyl]-N-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}-1,3,5-triazine-2,4-diamine. 